PDBsum entry 5l4k

Structural protein

PDB id: 5l4k

Contents

Protein chains

196 a.a.

293 a.a.

272 a.a.

56 a.a.

881 a.a.

876 a.a.

491 a.a.

456 a.a.

422 a.a.

389 a.a.

292 a.a.

376 a.a.

PDB id:

5l4k

Name:

Structural protein

Title:

The human 26s proteasome lid

Structure:

26s proteasome non-atpase regulatory subunit 4. Chain: w. Synonym: 26s proteasome regulatory subunit rpn10,26s proteasome regulatory subunit s5a,antisecretory factor 1,asf,multiubiquitin chain-binding protein. 26s proteasome non-atpase regulatory subunit 14. Chain: v. Synonym: 26s proteasome regulatory subunit rpn11,26s proteasome- associated pad1 homolog 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606

Authors:

A.Schweitzer,A.Aufderheide,T.Rudack,F.Beck

Key ref:

A.Schweitzer et al. (2016). Structure of the human 26S proteasome at a resolution of 3.9 Å. Proc Natl Acad Sci U S A, 113, 7816-7821. PubMed id: 27342858 DOI: 10.1073/pnas.1608050113

Date:

25-May-16 Release date: 07-Sep-16

Protein chain

P55036  (PSMD4_HUMAN) -  26S proteasome non-ATPase regulatory subunit 4 from Homo sapiens

Seq: 377 a.a.

Struc: 196 a.a.

Protein chain

O00487  (PSDE_HUMAN) -  26S proteasome non-ATPase regulatory subunit 14 from Homo sapiens

Seq: 310 a.a.

Struc: 293 a.a.

Protein chain

P48556  (PSMD8_HUMAN) -  26S proteasome non-ATPase regulatory subunit 8 from Homo sapiens

Seq: 350 a.a.

Struc: 272 a.a.

Protein chain

P60896  (SEM1_HUMAN) -  26S proteasome complex subunit SEM1 from Homo sapiens

Seq: 70 a.a.

Struc: 56 a.a.

Protein chain

Q13200  (PSMD2_HUMAN) -  26S proteasome non-ATPase regulatory subunit 2 from Homo sapiens

Seq: 908 a.a.

Struc: 881 a.a.

Protein chain

Q99460  (PSMD1_HUMAN) -  26S proteasome non-ATPase regulatory subunit 1 from Homo sapiens

Seq: 953 a.a.

Struc: 876 a.a.

Protein chain

O43242  (PSMD3_HUMAN) -  26S proteasome non-ATPase regulatory subunit 3 from Homo sapiens

Seq: 534 a.a.

Struc: 491 a.a.

Protein chain

O00232  (PSD12_HUMAN) -  26S proteasome non-ATPase regulatory subunit 12 from Homo sapiens

Seq: 456 a.a.

Struc: 456 a.a.

Protein chain

O00231  (PSD11_HUMAN) -  26S proteasome non-ATPase regulatory subunit 11 from Homo sapiens

Seq: 422 a.a.

Struc: 422 a.a.

Protein chain

Q15008  (PSMD6_HUMAN) -  26S proteasome non-ATPase regulatory subunit 6 from Homo sapiens

Seq: 389 a.a.

Struc: 389 a.a.

Protein chain

P51665  (PSMD7_HUMAN) -  26S proteasome non-ATPase regulatory subunit 7 from Homo sapiens

Seq: 324 a.a.

Struc: 292 a.a.

Protein chain

Q9UNM6  (PSD13_HUMAN) -  26S proteasome non-ATPase regulatory subunit 13 from Homo sapiens

Seq: 376 a.a.

Struc: 376 a.a.

Enzyme reactions

• Enzyme class: Chain V: E.C.3.4.19.- - ?????

DOI no: 10.1073/pnas.1608050113

Proc Natl Acad Sci U S A 113:7816-7821 (2016)

PubMed id: 27342858

Structure of the human 26S proteasome at a resolution of 3.9 Å.

A.Schweitzer, A.Aufderheide, T.Rudack, F.Beck, G.Pfeifer, J.M.Plitzko, E.Sakata, K.Schulten, F.Förster, W.Baumeister.

ABSTRACT

Protein degradation in eukaryotic cells is performed by the Ubiquitin-Proteasome System (UPS). The 26S proteasome holocomplex consists of a core particle (CP) that proteolytically degrades polyubiquitylated proteins, and a regulatory particle (RP) containing the AAA-ATPase module. This module controls access to the proteolytic chamber inside the CP and is surrounded by non-ATPase subunits (Rpns) that recognize substrates and deubiquitylate them before unfolding and degradation. The architecture of the 26S holocomplex is highly conserved between yeast and humans. The structure of the human 26S holocomplex described here reveals previously unidentified features of the AAA-ATPase heterohexamer. One subunit, Rpt6, has ADP bound, whereas the other five have ATP in their binding pockets. Rpt6 is structurally distinct from the other five Rpt subunits, most notably in its pore loop region. For Rpns, the map reveals two main, previously undetected, features: the C terminus of Rpn3 protrudes into the mouth of the ATPase ring; and Rpn1 and Rpn2, the largest proteasome subunits, are linked by an extended connection. The structural features of the 26S proteasome observed in this study are likely to be important for coordinating the proteasomal subunits during substrate processing.