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PDBsum entry 5l0m
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Transcription/DNA
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PDB id
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5l0m
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PDB id:
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| Name: |
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Transcription/DNA
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Title:
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Hlrh-1 DNA binding domain - 12bp oct4 promoter complex
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Structure:
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Nuclear receptor subfamily 5 group a member 2. Chain: a. Fragment: unp residues 79-187. Synonym: alpha-1-fetoprotein transcription factor,b1-binding factor, hb1f,cyp7a promoter-binding factor,hepatocytic transcription factor, liver receptor homolog 1,lrh-1. Engineered: yes. DNA (5'-d( Cp Tp Ap Gp Cp Cp Tp Tp Gp Ap Cp C)-3'). Chain: c.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: nr5a2, b1f, cpf, ftf. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Mus musculus. Organism_taxid: 10090.
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Resolution:
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2.20Å
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R-factor:
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0.158
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R-free:
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0.197
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Authors:
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M.L.Tuntland,E.A.Ortlund
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Key ref:
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E.R.Weikum
et al.
(2016).
A Structural Investigation into Oct4 Regulation by Orphan Nuclear Receptors, Germ Cell Nuclear Factor (GCNF), and Liver Receptor Homolog-1 (LRH-1).
J Mol Biol,
428,
4981-4992.
PubMed id:
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Date:
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27-Jul-16
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Release date:
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09-Nov-16
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PROCHECK
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Headers
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References
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O00482
(NR5A2_HUMAN) -
Nuclear receptor subfamily 5 group A member 2 from Homo sapiens
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Seq:
Struc:
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541 a.a.
96 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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C-T-A-G-C-C-T-T-G-A-C-C
12 bases
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G-G-T-C-A-A-G-G-C-T-A-G
12 bases
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J Mol Biol
428:4981-4992
(2016)
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PubMed id:
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A Structural Investigation into Oct4 Regulation by Orphan Nuclear Receptors, Germ Cell Nuclear Factor (GCNF), and Liver Receptor Homolog-1 (LRH-1).
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E.R.Weikum,
M.L.Tuntland,
M.N.Murphy,
E.A.Ortlund.
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ABSTRACT
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Oct4 is a transcription factor required for maintaining pluripotency and
self-renewal in stem cells. Prior to differentiation, Oct4 must be silenced to
allow for the development of the three germ layers in the developing embryo.
This fine-tuning is controlled by the nuclear receptors (NRs), liver receptor
homolog-1 (LRH-1) and germ cell nuclear factor (GCNF). Liver receptor homolog-1
is responsible for driving the expression of Oct4 where GCNF represses its
expression upon differentiation. Both receptors bind to a DR0 motif located
within the Oct4 promoter. Here, we present the first structure of mouse GCNF
DNA-binding domain in complex with the Oct4 DR0. The overall structure revealed
two molecules bound in a head-to-tail fashion on opposite sides of the DNA.
Additionally, we solved the structure of the human LRH-1 DNA-binding domain
bound to the same element. We explore the structural elements that govern Oct4
recognition by these two NRs.
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Links
