PDBsum entry 5kj7

Endocytosis,exocytosis

PDB id: 5kj7

Contents

Protein chains

63 a.a.

66 a.a.

74 a.a.

64 a.a.

279 a.a.

Metals

_CA ×19

Waters ×17

PDB id:

5kj7

Name:

Endocytosis,exocytosis

Title:

Structure of the ca2+-bound synaptotagmin-1 snare complex (long unit cell form) - from xfel diffraction

Structure:

Vesicle-associated membrane protein 3. Chain: a, g. Fragment: unp residues 14-76. Synonym: vamp-3,cellubrevin,ceb,synaptobrevin-3. Engineered: yes. Other_details: 96% helical (1 helices. 61 residues). Syntaxin-1a. Chain: b, h. Fragment: unp residues 191-256.

Source:

Rattus norvegicus. Rat. Organism_taxid: 10116. Gene: vamp3, syb3. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: stx1a, sap. Gene: snap25, snap. Gene: syt1.

Resolution:

3.50Å R-factor: 0.294 R-free: 0.329

Authors:

A.Y.Lyubimov,M.Uervirojnangkoorn,Q.Zhou,M.Zhao,N.K.Sauter, A.S.Brewster,W.I.Weis,A.T.Brunger

Key ref:

A.Y.Lyubimov et al. (2016). Advances in X-Ray free electron laser (xfel) diffract data processing applied to the crystal structure of t synaptotagmin-1 / snare complex.. Elife, 5, . PubMed id: 27731796

Date:

17-Jun-16 Release date: 19-Oct-16

Protein chains

P63025  (VAMP3_RAT) -  Vesicle-associated membrane protein 3 from Rattus norvegicus

Seq: 103 a.a.

Struc: 63 a.a.*

Protein chains

P32851  (STX1A_RAT) -  Syntaxin-1A from Rattus norvegicus

Seq: 288 a.a.

Struc: 66 a.a.

Protein chains

P60881  (SNP25_RAT) -  Synaptosomal-associated protein 25 from Rattus norvegicus

Seq: 206 a.a.

Struc: 74 a.a.*

Protein chains

P60881  (SNP25_RAT) -  Synaptosomal-associated protein 25 from Rattus norvegicus

Seq: 206 a.a.

Struc: 64 a.a.

Protein chains

P21707  (SYT1_RAT) -  Synaptotagmin-1 from Rattus norvegicus

Seq: 421 a.a.

Struc: 279 a.a.

* PDB and UniProt seqs differ at 7 residue positions (black crosses)