PDBsum entry 5kho

Signaling protein

PDB id: 5kho

Contents

Protein chains

103 a.a.

165 a.a.

Ligands

GOL

GNP ×2

Metals

_MG ×2

Waters ×14

PDB id:

5kho

Name:

Signaling protein

Title:

Rasip1 ra domain in complex with rap1b

Structure:

Ras-interacting protein 1. Chain: a, b. Synonym: rain. Engineered: yes. Ras-related protein rap-1b. Chain: c, d. Synonym: gtp-binding protein smg p21b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rasip1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: rap1b, ok/sw-cl.11.

Resolution:

2.78Å R-factor: 0.209 R-free: 0.286

Authors:

A.R.Gingras

Key ref:

A.R.Gingras et al. (2016). Structural Basis of Dimeric Rasip1 RA Domain Recognition of the Ras Subfamily of GTP-Binding Proteins. Structure, 24, 2152-2162. PubMed id: 27839947 DOI: 10.1016/j.str.2016.10.001

Date:

15-Jun-16 Release date: 19-Oct-16

Protein chains

Q5U651  (RAIN_HUMAN) -  Ras-interacting protein 1 from Homo sapiens

Seq: 963 a.a.

Struc: 103 a.a.

Protein chains

P61224  (RAP1B_HUMAN) -  Ras-related protein Rap-1b from Homo sapiens

Seq: 184 a.a.

Struc: 165 a.a.

Enzyme reactions

• Enzyme class: Chains C, D: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GNP) matches with 81.82% similarity) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.str.2016.10.001

Structure 24:2152-2162 (2016)

PubMed id: 27839947

Structural Basis of Dimeric Rasip1 RA Domain Recognition of the Ras Subfamily of GTP-Binding Proteins.

A.R.Gingras, W.Puzon-McLaughlin, A.A.Bobkov, M.H.Ginsberg.

ABSTRACT

No abstract given.