PDBsum entry 5ke9

Transcription factor/DNA

PDB id: 5ke9

Contents

Protein chain

88 a.a.

DNA/RNA

Metals

_ZN ×3

Waters ×14

PDB id:

5ke9

Name:

Transcription factor/DNA

Title:

Mouse klf4 e446p znf1-3 and tpg/cpa sequence DNA complex structure

Structure:

Krueppel-like factor 4. Chain: a. Fragment: unp residues 396-483. Synonym: epithelial zinc finger protein ezf,gut-enriched krueppel- like factor. Engineered: yes. Mutation: yes. DNA (5'-d( Gp Ap Gp Gp Tp Gp Tp Gp Gp C)-3'). Chain: b.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: klf4, ezf, gklf, zie. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

2.34Å R-factor: 0.217 R-free: 0.272

Authors:

H.Hashimoto,X.Cheng

Key ref:

H.Hashimoto et al. (2016). Distinctive Klf4 mutants determine preference for DNA methylation status. Nucleic Acids Res, 44, 10177-10185. PubMed id: 27596594 DOI: 10.1093/nar/gkw774

Date:

09-Jun-16 Release date: 14-Sep-16

Protein chain

Q60793  (KLF4_MOUSE) -  Krueppel-like factor 4 from Mus musculus

Seq: 483 a.a.

Struc: 88 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-A-G-G-T-G-T-G-G-C 10 bases

G-C-C-A-C-A-C-C-T-C 10 bases

DOI no: 10.1093/nar/gkw774

Nucleic Acids Res 44:10177-10185 (2016)

PubMed id: 27596594

Distinctive Klf4 mutants determine preference for DNA methylation status.

H.Hashimoto, D.Wang, A.N.Steves, P.Jin, R.M.Blumenthal, X.Zhang, X.Cheng.

ABSTRACT

No abstract given.