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PDBsum entry 5ke8
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protein dna_rna metals links
Transcription/DNA PDB id
5ke8

 

 

 

 

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Contents
Protein chain
85 a.a.
DNA/RNA
Metals
_ZN ×3
Waters ×9
PDB id:
5ke8
Name: Transcription/DNA
Title: Mouse klf4 e446p znf1-3 and mpg/mpg sequence DNA complex structure
Structure: Krueppel-like factor 4. Chain: a. Fragment: unp residues 396-483. Synonym: epithelial zinc finger protein ezf,gut-enriched krueppel- like factor. Engineered: yes. Mutation: yes. DNA (5'-d( Gp Ap Gp Gp (5Cm)p Gp Tp Gp Gp C)-3'). Chain: b.
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: klf4, ezf, gklf, zie. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.
Resolution:
2.45Å     R-factor:   0.233     R-free:   0.270
Authors: H.Hashimoto,X.Cheng
Key ref: H.Hashimoto et al. (2016). Distinctive Klf4 mutants determine preference for DNA methylation status. Nucleic Acids Res, 44, 10177-10185. PubMed id: 27596594 DOI: 10.1093/nar/gkw774
Date:
09-Jun-16     Release date:   14-Sep-16    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q60793  (KLF4_MOUSE) -  Krueppel-like factor 4 from Mus musculus
Seq:
Struc: 		483 a.a.
85 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains
  G-A-G-G-5CM-G-T-G-G-C 10 bases
  G-C-C-A-5CM-G-C-C-T-C 10 bases

 

 
DOI no: 10.1093/nar/gkw774 Nucleic Acids Res 44:10177-10185 (2016)
PubMed id: 27596594  
 
 
Distinctive Klf4 mutants determine preference for DNA methylation status.
H.Hashimoto, D.Wang, A.N.Steves, P.Jin, R.M.Blumenthal, X.Zhang, X.Cheng.
 
  ABSTRACT  
 
No abstract given.

 

 

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