PDBsum entry 5k8s

Transferase

PDB id: 5k8s

Contents

Protein chains

146 a.a.

Ligands

CMP ×2

Waters ×356

PDB id:

5k8s

Name:

Transferase

Title:

Camp bound pfpka-r (297-441)

Structure:

Camp-dependent protein kinase regulatory subunit. Chain: a, b. Fragment: unp residues 297-441. Engineered: yes

Source:

Plasmodium falciparum (isolate 3d7). Organism_taxid: 36329. Strain: isolate 3d7. Gene: pkar, pfl1110c. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.15Å R-factor: 0.199 R-free: 0.218

Authors:

D.R.Littler,P.R.Gilson,B.S.Crabb,J.J.Rossjohn

Key ref:

D.R.Littler et al. (2016). Disrupting the Allosteric Interaction between the Plasmodium falciparum cAMP-dependent Kinase and Its Regulatory Subunit. J Biol Chem, 291, 25375-25386. PubMed id: 27738107 DOI: 10.1074/jbc.M116.750174

Date:

31-May-16 Release date: 12-Oct-16

Protein chains

Q7KQK0  (Q7KQK0_PLAF7) -  cAMP-dependent protein kinase regulatory subunit from Plasmodium falciparum (isolate 3D7)

Seq: 441 a.a.

Struc: 146 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.11.11 - cAMP-dependent protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = CMP) matches with 81.48% similarity) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = CMP) matches with 81.48% similarity) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1074/jbc.M116.750174

J Biol Chem 291:25375-25386 (2016)

PubMed id: 27738107

Disrupting the Allosteric Interaction between the Plasmodium falciparum cAMP-dependent Kinase and Its Regulatory Subunit.

D.R.Littler, H.E.Bullen, K.L.Harvey, T.Beddoe, B.S.Crabb, J.Rossjohn, P.R.Gilson.

ABSTRACT

No abstract given.