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PDBsum entry 5jxb
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Cell adhesion
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PDB id
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5jxb
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References listed in PDB file
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Key reference
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Title
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Phase transition in postsynaptic densities underlies formation of synaptic complexes and synaptic plasticity.
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Authors
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M.Zeng,
Y.Shang,
Y.Araki,
T.Guo,
R.L.Huganir,
M.Zhang.
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Ref.
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Cell, 2016,
166,
1163.
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PubMed id
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Abstract
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Postsynaptic densities (PSDs) are membrane semi-enclosed, submicron
protein-enriched cellular compartments beneath postsynaptic membranes, which
constantly exchange their components with bulk aqueous cytoplasm in synaptic
spines. Formation and activity-dependent modulation of PSDs is considered as one
of the most basic molecular events governing synaptic plasticity in the nervous
system. In this study, we discover that SynGAP, one of the most abundant PSD
proteins and a Ras/Rap GTPase activator, forms a homo-trimer and binds to
multiple copies of PSD-95. Binding of SynGAP to PSD-95 induces phase separation
of the complex, forming highly concentrated liquid-like droplets reminiscent of
the PSD. The multivalent nature of the SynGAP/PSD-95 complex is critical for the
phase separation to occur and for proper activity-dependent SynGAP dispersions
from the PSD. In addition to revealing a dynamic anchoring mechanism of SynGAP
at the PSD, our results also suggest a model for phase-transition-mediated
formation of PSD.
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