PDBsum entry 5jqp

Hydrolase

PDB id: 5jqp

Contents

Protein chains

918 a.a.

131 a.a.

Ligands

TRS

Metals

_CA ×2

Waters ×655

PDB id:

5jqp

Name:

Hydrolase

Title:

Crystal structure of er glucosidase ii heterodimeric complex consisting of catalytic subunit and the binding domain of regulatory subunit

Structure:

Alpha glucosidase-like protein. Chain: a. Engineered: yes. Glucosidase 2 subunit beta-like protein. Chain: b. Fragment: unp residues 21-162. Engineered: yes

Source:

Chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719). Organism_taxid: 759272. Strain: dsm 1495 / cbs 144.50 / imi 039719. Gene: ctht_0064960. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: ctht_0046400.

Resolution:

2.20Å R-factor: 0.155 R-free: 0.206

Authors:

T.Satoh,T.Toshimori,M.Noda,S.Uchiyama,K.Kato

Key ref:

T.Satoh et al. (2016). Interaction mode between catalytic and regulatory subunits in glucosidase II involved in ER glycoprotein quality control. Protein Sci, 25, 2095-2101. PubMed id: 27576940 DOI: 10.1002/pro.3031

Date:

05-May-16 Release date: 14-Sep-16

Protein chain

G0SG42  (G0SG42_CHATD) -  alpha-glucosidase from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 977 a.a.

Struc: 918 a.a.

Protein chain

G0S9M2  (G0S9M2_CHATD) -  Glucosidase 2 subunit beta from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 562 a.a.

Struc: 131 a.a.

Enzyme reactions

• Enzyme class: Chain A: E.C.3.2.1.20 - alpha-glucosidase.
• Reaction: Hydrolysis of terminal, non-reducing 1,4-linked D-glucose residues with release of D-glucose.

DOI no: 10.1002/pro.3031

Protein Sci 25:2095-2101 (2016)

PubMed id: 27576940

Interaction mode between catalytic and regulatory subunits in glucosidase II involved in ER glycoprotein quality control.

T.Satoh, T.Toshimori, M.Noda, S.Uchiyama, K.Kato.

ABSTRACT

No abstract given.