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PDBsum entry 5jk9
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Cell adhesion
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PDB id
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5jk9
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References listed in PDB file
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Key reference
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Title
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Structure of izumo1-Juno reveals sperm-Oocyte recognition during mammalian fertilization.
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Authors
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U.Ohto,
H.Ishida,
E.Krayukhina,
S.Uchiyama,
N.Inoue,
T.Shimizu.
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Ref.
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Nature, 2016,
534,
566-569.
[DOI no: ]
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PubMed id
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Abstract
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Fertilization is a fundamental process in sexual reproduction, creating a new
individual through the combination of male and female gametes. The IZUMO1 sperm
membrane protein and its counterpart oocyte receptor JUNO have been identified
as essential factors for sperm-oocyte interaction and fusion. However, the
mechanism underlying their specific recognition remains poorly defined. Here, we
show the crystal structures of human IZUMO1, JUNO and the IZUMO1-JUNO complex,
establishing the structural basis for the IZUMO1-JUNO-mediated sperm-oocyte
interaction. IZUMO1 exhibits an elongated rod-shaped structure comprised of a
helical bundle IZUMO domain and an immunoglobulin-like domain that are each
firmly anchored to an intervening β-hairpin region through conserved disulfide
bonds. The central β-hairpin region of IZUMO1 provides the main platform for
JUNO binding, while the surface located behind the putative JUNO ligand binding
pocket is involved in IZUMO1 binding. Structure-based mutagenesis analysis
confirms the biological importance of the IZUMO1-JUNO interaction. This
structure provides a major step towards elucidating an essential phase of
fertilization and it will contribute to the development of new therapeutic
interventions for fertility, such as contraceptive agents.
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