PDBsum entry 5jjw

RNA-binding protein/hydrolase

PDB id: 5jjw

Contents

Protein chains

296 a.a.

149 a.a.

Ligands

EDO

UNX

Waters ×11

PDB id:

5jjw

Name:

RNA-binding protein/hydrolase

Title:

Crystal structure of the hat domain of sart3 in complex with usp15 dusp-ubl domain

Structure:

Squamous cell carcinoma antigen recognized by t-cells 3. Chain: a. Fragment: unp residues 280-578. Synonym: sart-3,tat-interacting protein of 110 kda,tip110,p110 nuclear RNA-binding protein. Engineered: yes. Ubiquitin carboxyl-terminal hydrolase 15. Chain: b. Fragment: unp residues 1-223.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sart3, kiaa0156, tip110. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: usp15, kiaa0529.

Resolution:

3.01Å R-factor: 0.237 R-free: 0.288

Authors:

A.Dong,Q.Zhang,J.R.Walker,C.Bountra,C.H.Arrowsmith,A.M.Edwards, Y.Tong,Structural Genomics Consortium (Sgc)

Key ref:

H.Wu et al. Crystal structure of pias3. To be published, .

Date:

25-Apr-16 Release date: 04-May-16

Protein chain

Q15020  (SART3_HUMAN) -  Squamous cell carcinoma antigen recognized by T-cells 3 from Homo sapiens

Seq: 963 a.a.

Struc: 296 a.a.

Protein chain

Q9Y4E8  (UBP15_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 15 from Homo sapiens

Seq: 981 a.a.

Struc: 149 a.a.*

* PDB and UniProt seqs differ at 9 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).