PDBsum entry 5jjk

Transcription/RNA

PDB id: 5jjk

Contents

Protein chains

405 a.a.

356 a.a.

DNA/RNA

Ligands

ADP ×6

BEF ×6

Metals

_MG ×6

Waters ×19

PDB id:

5jjk

Name:

Transcription/RNA

Title:

Rho transcription termination factor bound to ra7 and 6 adp-bef3 molecules

Structure:

Transcription termination factor rho. Chain: a, b, c, d, e, f. Synonym: atp-dependent helicase rho. Engineered: yes. Mutation: yes. Ra12: RNA (5'-r(p Ap Ap Ap Ap Ap Ap A)-3'). Chain: g. Engineered: yes

Source:

Escherichia coli o157:h7. Organism_taxid: 83334. Gene: rho, z5293, ecs4716. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

3.15Å R-factor: 0.235 R-free: 0.265

Authors:

N.D.Thomsen,M.R.Lawson,L.B.Witkowsky,S.Qu,J.M.Berger

Key ref:

N.D.Thomsen et al. (2016). Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor. Proc Natl Acad Sci U S A, 113, E7691. PubMed id: 27856760 DOI: 10.1073/pnas.1616745113

Date:

24-Apr-16 Release date: 16-Nov-16

Protein chains

P0AG30  (RHO_ECOLI) -  Transcription termination factor Rho from Escherichia coli (strain K12)

Seq: 419 a.a.

Struc: 405 a.a.

Protein chain

P0AG30  (RHO_ECOLI) -  Transcription termination factor Rho from Escherichia coli (strain K12)

Seq: 419 a.a.

Struc: 356 a.a.

DNA/RNA chain

A-A-A-A-A-A-A 7 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.3.6.4.- - ?????

DOI no: 10.1073/pnas.1616745113

Proc Natl Acad Sci U S A 113:E7691 (2016)

PubMed id: 27856760

Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor.

N.D.Thomsen, M.R.Lawson, L.B.Witkowsky, S.Qu, J.M.Berger.

ABSTRACT

No abstract given.