PDBsum entry 5jbt

Hydrolase/hydrolase inhibitor

PDB id: 5jbt

Contents

Protein chains

224 a.a.

14 a.a.

38 a.a.

Ligands

SO4

Metals

_CA

Waters ×261

PDB id:

5jbt

Name:

Hydrolase/hydrolase inhibitor

Title:

Mesotrypsin in complex with cleaved amyloid precursor like protein 2 inhibitor (aplp2)

Structure:

Prss3 protein. Chain: a. Engineered: yes. Mutation: yes. Amyloid-like protein 2. Chain: x. Synonym: aplp-2,apph,amyloid protein homolog,cdei box-binding protein,cdebp. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organ: pancreas. Gene: prss3. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: aplp2, appl2. Expressed in: komagataella pastoris.

Resolution:

1.40Å R-factor: 0.165 R-free: 0.199

Authors:

O.Kayode,R.Wang,D.Pendlebury,A.Soares,E.S.Radisky

Key ref:

O.Kayode et al. (2016). An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis. J Biol Chem, 291, 26304-26319. PubMed id: 27810896 DOI: 10.1074/jbc.M116.758417

Date:

13-Apr-16 Release date: 09-Nov-16

Protein chain

P35030  (TRY3_HUMAN) -  Trypsin-3 from Homo sapiens

Seq: 304 a.a.

Struc: 224 a.a.*

Protein chain

Q06481  (APLP2_HUMAN) -  Amyloid beta precursor like protein 2 from Homo sapiens

Seq: 763 a.a.

Struc: 14 a.a.

Protein chain

Q06481  (APLP2_HUMAN) -  Amyloid beta precursor like protein 2 from Homo sapiens

Seq: 763 a.a.

Struc: 38 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

DOI no: 10.1074/jbc.M116.758417

J Biol Chem 291:26304-26319 (2016)

PubMed id: 27810896

An Acrobatic Substrate Metamorphosis Reveals a Requirement for Substrate Conformational Dynamics in Trypsin Proteolysis.

O.Kayode, R.Wang, D.F.Pendlebury, I.Cohen, R.D.Henin, A.Hockla, A.S.Soares, N.Papo, T.R.Caulfield, E.S.Radisky.

ABSTRACT

No abstract given.