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PDBsum entry 5j8y
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Nuclear protein
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PDB id
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5j8y
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References listed in PDB file
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Key reference
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Title
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Molecular basis of prc1 targeting to polycomb response elements by phorc.
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Authors
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F.Frey,
T.Sheahan,
K.Finkl,
G.Stoehr,
M.Mann,
C.Benda,
J.Müller.
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Ref.
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Genes Dev, 2016,
30,
1116-1127.
[DOI no: ]
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PubMed id
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Abstract
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Polycomb group (PcG) protein complexes repress transcription by modifying target
gene chromatin. In Drosophila, this repression requires association of PcG
protein complexes with cis-regulatory Polycomb response elements (PREs), but the
interactions permitting formation of these assemblies are poorly understood. We
show that the Sfmbt subunit of the DNA-binding Pho-repressive complex (PhoRC)
and the Scm subunit of the canonical Polycomb-repressive complex 1 (PRC1)
directly bind each other through their SAM domains. The 1.9 Å crystal structure
of the Scm-SAM:Sfmbt-SAM complex reveals the recognition mechanism and shows
that Sfmbt-SAM lacks the polymerization capacity of the SAM domains of Scm and
its PRC1 partner subunit, Ph. Functional analyses in Drosophila demonstrate that
Sfmbt-SAM and Scm-SAM are essential for repression and that PhoRC DNA binding is
critical to initiate PRC1 association with PREs. Together, this suggests that
PRE-tethered Sfmbt-SAM nucleates PRC1 recruitment and that
Scm-SAM/Ph-SAM-mediated polymerization then results in the formation of
PRC1-compacted chromatin.
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