PDBsum entry 5j7c

Protein binding/hydrolase

PDB id: 5j7c

Contents

Protein chains

129 a.a.

97 a.a.

Waters ×52

PDB id:

5j7c

Name:

Protein binding/hydrolase

Title:

A picomolar affinity fn3 domain in complex with hen egg-white lysozyme

Structure:

LysozymE C. Chain: a, b. Synonym: 1,4-beta-n-acetylmuramidasE C,allergen gal d iv. Fnfn10-anti-lysozyme (de0.4.1). Chain: c, d. Engineered: yes

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.54Å R-factor: 0.219 R-free: 0.250

Authors:

B.T.Porebski,N.Drinkwater,S.Mcgowan,A.M.Buckle

Key ref:

B.T.Porebski et al. Circumventing the stability-Function trade-Off in an engineered fn3 domain. Protein eng des sel, . PubMed id: 27578887

Date:

06-Apr-16 Release date: 17-Aug-16

Protein chains

P00698  (LYSC_CHICK) -  Lysozyme C from Gallus gallus

Seq: 147 a.a.

Struc: 129 a.a.

Protein chains

P02751  (FINC_HUMAN) -  Fibronectin from Homo sapiens

Seq: 2477 a.a.

Struc: 97 a.a.*

* PDB and UniProt seqs differ at 27 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.