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PDBsum entry 5j1i
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Structural protein
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PDB id
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5j1i
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PDB id:
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| Name: |
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Structural protein
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Title:
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Structure of the spectrin repeats 7, 8, and 9 of the plakin domain of plectin
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Structure:
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Plectin. Chain: a, b. Fragment: unp residues 1104-1372. Synonym: pltn,hemidesmosomal protein 1,hd1,plectin-1. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: plec, plec1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Resolution:
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2.80Å
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R-factor:
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0.297
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R-free:
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0.322
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Authors:
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E.Ortega,J.M.De Pereda
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Key ref:
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E.Ortega
et al.
(2016).
The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.
J Biol Chem,
291,
18643-18662.
PubMed id:
DOI:
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Date:
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29-Mar-16
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Release date:
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20-Jul-16
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PROCHECK
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Headers
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References
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Q15149
(PLEC_HUMAN) -
Plectin from Homo sapiens
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Seq:
Struc:
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4684 a.a.
347 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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DOI no:
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J Biol Chem
291:18643-18662
(2016)
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PubMed id:
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The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.
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E.Ortega,
J.A.Manso,
R.M.Buey,
A.M.Carballido,
A.Carabias,
A.Sonnenberg,
J.M.de Pereda.
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ABSTRACT
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Plakins are large multi-domain proteins that interconnect cytoskeletal
structures. Plectin is a prototypical plakin that tethers intermediate filaments
to membrane-associated complexes. Most plakins contain a plakin domain formed by
up to nine spectrin repeats (SR1-SR9) and an SH3 domain. The plakin domains of
plectin and other plakins harbor binding sites for junctional proteins. We have
combined x-ray crystallography with small angle x-ray scattering (SAXS) to
elucidate the structure of the plakin domain of plectin, extending our previous
analysis of the SR1 to SR5 region. Two crystal structures of the SR5-SR6 region
allowed us to characterize its uniquely wide inter-repeat conformational
variability. We also report the crystal structures of the SR7-SR8 region,
refined to 1.8 Å, and the SR7-SR9 at lower resolution. The SR7-SR9 region,
which is conserved in all other plakin domains, forms a rigid segment stabilized
by uniquely extensive inter-repeat contacts mediated by unusually long helices
in SR8 and SR9. Using SAXS we show that in solution the SR3-SR6 and SR7-SR9
regions are rod-like segments and that SR3-SR9 of plectin has an extended shape
with a small central kink. Other plakins, such as bullous pemphigoid antigen 1
and microtubule and actin cross-linking factor 1, are likely to have similar
extended plakin domains. In contrast, desmoplakin has a two-segment structure
with a central flexible hinge. The continuous versus segmented structures of the
plakin domains of plectin and desmoplakin give insight into how different
plakins might respond to tension and transmit mechanical signals.
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