PDBsum entry 5int

Ligase

PDB id: 5int

Contents

Protein chains

196 a.a.

198 a.a.

Waters ×244

PDB id:

5int

Name:

Ligase

Title:

Crystal structure of thE C-terminal domain of coenzyme a biosynthesis bifunctional protein coabc

Structure:

Phosphopantothenate--cysteine ligase. Chain: a, b. Synonym: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase,probable coenzyme a biosynthesis bifunctional protein coabc. Engineered: yes

Source:

Bacillus anthracis. Organism_taxid: 1392. Gene: coabc, gbaa_4007, ab163_23415, ab164_18935, ab165_13850, ab166_05815, ab167_23710, ab168_27015, ab169_03120, ab170_11765, ab171_07935, ab893_19460, adk17_20550, adt20_07585, adt21_16720, bash2_01930. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.15Å R-factor: 0.227 R-free: 0.258

Authors:

B.Nocek,M.Zhou,S.Grimshaw,Y.Kim,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

B.Nocek et al. Crystal structure of the c-Terminal domain of coenzym biosynthesis bifunctional protein coabc. To be published, .

Date:

07-Mar-16 Release date: 06-Apr-16

Protein chain

A0A6L8Q0G8  (A0A6L8Q0G8_BACAN) -  Coenzyme A biosynthesis bifunctional protein CoaBC from Bacillus anthracis

Seq: 401 a.a.

Struc: 196 a.a.*

Protein chain

A0A6L8Q0G8  (A0A6L8Q0G8_BACAN) -  Coenzyme A biosynthesis bifunctional protein CoaBC from Bacillus anthracis

Seq: 401 a.a.

Struc: 198 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.4.1.1.36 - phosphopantothenoylcysteine decarboxylase.
• Pathway: Coenzyme A Biosynthesis (late stages)
• Reaction: N-[(R)-4-phosphopantothenoyl]-L-cysteine + H⁺ = (R)- 4'-phosphopantetheine + CO2
• Cofactor: FMN
• Enzyme class 2: Chains A, B: E.C.6.3.2.5 - phosphopantothenate--cysteine ligase (CTP).

Pathway:

• Reaction: (R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4- phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site