PDBsum entry 5ifs

Signaling protein

PDB id: 5ifs

Contents

Protein chains

181 a.a.

444 a.a.

Ligands

ADP ×2

SO4

EDO ×3

GOL

Metals

_MG ×2

Waters ×408

PDB id:

5ifs

Name:

Signaling protein

Title:

Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in aspl that disrupts functional p97 hexamers and induces cell death

Structure:

Tether containing ubx domain for glut4. Chain: a, c. Fragment: unp residues 317-553. Synonym: alveolar soft part sarcoma chromosomal region candidate gene 1 protein,alveolar soft part sarcoma locus,renal papillary cell carcinoma protein 17,ubx domain-containing protein 9. Engineered: yes. Transitional endoplasmic reticulum atpase. Chain: b, d.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: aspscr1, aspl, rcc17, tug, ubxd9, ubxn9. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta2. Gene: vcp. Expression_system_variant: rosetta 2.

Resolution:

2.46Å R-factor: 0.204 R-free: 0.251

Authors:

Y.Roske,A.Arumughan,U.Heinemann,E.Wanker

Key ref:

A.Arumughan et al. (2016). Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers. Nat Commun, 7, 13047. PubMed id: 27762274 DOI: 10.1038/ncomms13047

Date:

26-Feb-16 Release date: 26-Oct-16

Protein chains

Q9BZE9  (ASPC1_HUMAN) -  Tether containing UBX domain for GLUT4 from Homo sapiens

Seq: 553 a.a.

Struc: 181 a.a.

Protein chains

P55072  (TERA_HUMAN) -  Transitional endoplasmic reticulum ATPase from Homo sapiens

Seq: 806 a.a.

Struc: 444 a.a.

Enzyme reactions

• Enzyme class 1: Chains A, C: E.C.?
• Enzyme class 2: Chains B, D: E.C.3.6.4.6 - vesicle-fusing ATPase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1038/ncomms13047

Nat Commun 7:13047 (2016)

PubMed id: 27762274

Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers.

A.Arumughan, Y.Roske, C.Barth, L.L.Forero, K.Bravo-Rodriguez, A.Redel, S.Kostova, E.McShane, R.Opitz, K.Faelber, K.Rau, T.Mielke, O.Daumke, M.Selbach, E.Sanchez-Garcia, O.Rocks, D.Panáková, U.Heinemann, E.E.Wanker.

ABSTRACT

No abstract given.