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UniProt functional annotation for Q8BHN3 | ||
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| UniProt code: Q8BHN3. |
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| Organism: | |
Mus musculus (Mouse). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. |
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| Function: | |
Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:27462106). Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:28375157). {ECO:0000269|PubMed:27462106, ECO:0000269|PubMed:28375157}. |
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| Catalytic activity: | |
Reaction=H2O + N(4)-(alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man- (1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)- [alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D- Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc)-L-asparaginyl- [protein] = beta-D-glucose + N(4)-(alpha-D-Man-(1->2)-alpha-D-Man- (1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)- [alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D- Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc)-L-asparaginyl- [protein] (N-glucan mannose isomer 9A1,2,3B1,2,3); Xref=Rhea:RHEA:56000, Rhea:RHEA-COMP:14356, Rhea:RHEA-COMP:14357, ChEBI:CHEBI:15377, ChEBI:CHEBI:15903, ChEBI:CHEBI:59080, ChEBI:CHEBI:139493; EC=3.2.1.207; Evidence={ECO:0000269|PubMed:27462106}; |
| Catalytic activity: | |
Reaction=H2O + N(4)-(alpha-D-Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man- (1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)- alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D- Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc)-L- asparaginyl-[protein] = beta-D-glucose + N(4)-(alpha-D-Glc-(1->3)- alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D- Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man- (1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- alpha-D-GlcNAc)-L-asparaginyl-[protein]; Xref=Rhea:RHEA:55996, Rhea:RHEA-COMP:14355, Rhea:RHEA-COMP:14357, ChEBI:CHEBI:15377, ChEBI:CHEBI:15903, ChEBI:CHEBI:59080, ChEBI:CHEBI:59082; EC=3.2.1.207; Evidence={ECO:0000269|PubMed:27462106}; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 6.5 - 7.; |
| Pathway: | |
Glycan metabolism; N-glycan metabolism. {ECO:0000269|PubMed:27462106}. |
| Subunit: | |
Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH) (PubMed:9148925, PubMed:10921916, PubMed:27462106). Binds glycosylated PTPRC (PubMed:9148925, PubMed:10921916). {ECO:0000269|PubMed:10921916, ECO:0000269|PubMed:27462106, ECO:0000269|PubMed:9148925}. |
| Subcellular location: | |
Endoplasmic reticulum {ECO:0000250|UniProtKB:Q14697}. Golgi apparatus {ECO:0000250|UniProtKB:P79403}. Melanosome {ECO:0000250|UniProtKB:Q14697}. |
| Miscellaneous: | |
[Isoform 3]: May be due to an intron retention. {ECO:0000305}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 31 family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=BAC65483.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.