PDBsum entry 5ic9

Structural protein

PDB id: 5ic9

Contents

Protein chains

129 a.a.

132 a.a.

134 a.a.

103 a.a.

PDB id:

5ic9

Name:

Structural protein

Title:

Structure of the ctd complex of utp12 and utp13

Structure:

Putative uncharacterized protein. Chain: a, c. Fragment: unp residues 748-956. Synonym: u3 small nucleolar RNA-associated protein 12. Engineered: yes. Putative uncharacterized protein. Chain: b, d. Fragment: unp residues 738-912. Synonym: u3 small nucleolar RNA-associated protein 13.

Source:

Chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719). Organism_taxid: 759272. Strain: dsm 1495 / cbs 144.50 / imi 039719. Gene: ctht_0003430. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: ctht_0065530.

Resolution:

3.70Å R-factor: 0.277 R-free: 0.299

Authors:

C.Zhang,K.Ye

Key ref:

C.Zhang et al. (2016). Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits. Nucleic Acids Res, 44, 7475-7486. PubMed id: 27330138 DOI: 10.1093/nar/gkw562

Date:

23-Feb-16 Release date: 06-Jul-16

Protein chain

G0RZL9  (G0RZL9_CHATD) -  Small-subunit processome Utp12 domain-containing protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 962 a.a.

Struc: 129 a.a.

Protein chain

G0SG95  (G0SG95_CHATD) -  U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 912 a.a.

Struc: 132 a.a.

Protein chain

G0RZL9  (G0RZL9_CHATD) -  Small-subunit processome Utp12 domain-containing protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 962 a.a.

Struc: 134 a.a.

Protein chain

G0SG95  (G0SG95_CHATD) -  U3 small nucleolar RNA-associated protein 13 C-terminal domain-containing protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 912 a.a.

Struc: 103 a.a.

DOI no: 10.1093/nar/gkw562

Nucleic Acids Res 44:7475-7486 (2016)

PubMed id: 27330138

Integrative structural analysis of the UTPB complex, an early assembly factor for eukaryotic small ribosomal subunits.

C.Zhang, Q.Sun, R.Chen, X.Chen, J.Lin, K.Ye.

ABSTRACT

Ribosome assembly is an essential and conserved cellular process in eukaryotes that requires numerous assembly factors. The six-subunit UTPB complex is an essential component of the 90S precursor of the small ribosomal subunit. Here, we analyzed the molecular architecture of UTPB using an integrative structural biology approach. We mapped the major interactions that associate each of six UTPB proteins. Crystallographic studies showed that Utp1, Utp21, Utp12 and Utp13 are evolutionarily related and form a dimer of dimers (Utp1-Utp21, Utp12-Utp13) through their homologous helical C-terminal domains. Molecular docking with crosslinking restraints showed that the WD domains of Utp12 and Utp13 are associated, as are the WD domains of Utp1, Utp21 and Utp18. Electron microscopy images of the entire UTPB complex revealed that it predominantly adopts elongated conformations and possesses internal flexibility. We also determined crystal structures of the WD domain of Utp18 and the HAT and deviant HAT domains of Utp6. A structural model of UTPB was derived based on these data.