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PDBsum entry 5ic8
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Structural protein
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PDB id
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5ic8
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Contents |
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284 a.a.
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252 a.a.
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259 a.a.
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References listed in PDB file
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Key reference
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Title
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Integrative structural analysis of the utpb complex, An early assembly factor for eukaryotic small ribosomal subunits.
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Authors
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C.Zhang,
Q.Sun,
R.Chen,
X.Chen,
J.Lin,
K.Ye.
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Ref.
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Nucleic Acids Res, 2016,
44,
7475-7486.
[DOI no: ]
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PubMed id
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Abstract
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Ribosome assembly is an essential and conserved cellular process in eukaryotes
that requires numerous assembly factors. The six-subunit UTPB complex is an
essential component of the 90S precursor of the small ribosomal subunit. Here,
we analyzed the molecular architecture of UTPB using an integrative structural
biology approach. We mapped the major interactions that associate each of six
UTPB proteins. Crystallographic studies showed that Utp1, Utp21, Utp12 and Utp13
are evolutionarily related and form a dimer of dimers (Utp1-Utp21, Utp12-Utp13)
through their homologous helical C-terminal domains. Molecular docking with
crosslinking restraints showed that the WD domains of Utp12 and Utp13 are
associated, as are the WD domains of Utp1, Utp21 and Utp18. Electron microscopy
images of the entire UTPB complex revealed that it predominantly adopts
elongated conformations and possesses internal flexibility. We also determined
crystal structures of the WD domain of Utp18 and the HAT and deviant HAT domains
of Utp6. A structural model of UTPB was derived based on these data.
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