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PDBsum entry 5hyw
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Signaling protein/protein binding
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PDB id
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5hyw
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PDB id:
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| Name: |
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Signaling protein/protein binding
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Title:
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The crystal structure of the d3-ask1 complex
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Structure:
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F-box/lrr-repeat max2 homolog. Chain: a, c. Synonym: f-box and leucine-rich repeat max2 homolog,protein dwarf 3. Engineered: yes. Skp1-like protein 1a. Chain: b, d. Synonym: skp1-like 1,ufo-binding protein 1. Engineered: yes
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Source:
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Oryza sativa subsp. Japonica. Rice. Organism_taxid: 39947. Gene: d3, os06g0154200, loc_os06g06050, osjnba0085l11.6-1. Expressed in: insect cell expression vector ptie1. Expression_system_taxid: 266783. Arabidopsis thaliana. Mouse-ear cress. Organism_taxid: 3702.
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Resolution:
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3.01Å
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R-factor:
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0.239
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R-free:
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0.312
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Authors:
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R.F.Yao,Z.H.Ming,L.M.Yan,Z.H.Rao,Z.Y.Lou,D.X.Xie
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Key ref:
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R.Yao
et al.
(2016).
DWARF14 is a non-canonical hormone receptor for strigolactone.
Nature,
536,
469-473.
PubMed id:
DOI:
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Date:
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02-Feb-16
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Release date:
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03-Aug-16
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PROCHECK
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Headers
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References
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Q5VMP0
(MAX2_ORYSJ) -
F-box/LRR-repeat MAX2 homolog from Oryza sativa subsp. japonica
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Seq:
Struc:
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720 a.a.
580 a.a.
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DOI no:
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Nature
536:469-473
(2016)
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PubMed id:
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DWARF14 is a non-canonical hormone receptor for strigolactone.
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R.Yao,
Z.Ming,
L.Yan,
S.Li,
F.Wang,
S.Ma,
C.Yu,
M.Yang,
L.Chen,
L.Chen,
Y.Li,
C.Yan,
D.Miao,
Z.Sun,
J.Yan,
Y.Sun,
L.Wang,
J.Chu,
S.Fan,
W.He,
H.Deng,
F.Nan,
J.Li,
Z.Rao,
Z.Lou,
D.Xie.
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ABSTRACT
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Classical hormone receptors reversibly and non-covalently bind active hormone
molecules, which are generated by biosynthetic enzymes, to trigger signal
transduction. The α/β hydrolase DWARF14 (D14), which hydrolyses the plant
branching hormone strigolactone and interacts with the F-box protein D3/MAX2, is
probably involved in strigolactone detection. However, the active form of
strigolactone has yet to be identified and it is unclear which protein directly
binds the active form of strigolactone, and in which manner, to act as the
genuine strigolactone receptor. Here we report the crystal structure of the
strigolactone-induced AtD14-D3-ASK1 complex, reveal that Arabidopsis thaliana
(At)D14 undergoes an open-to-closed state transition to trigger strigolactone
signalling, and demonstrate that strigolactone is hydrolysed into a covalently
linked intermediate molecule (CLIM) to initiate a conformational change of AtD14
to facilitate interaction with D3. Notably, analyses of a highly branched
Arabidopsis mutant d14-5 show that the AtD14(G158E) mutant maintains enzyme
activity to hydrolyse strigolactone, but fails to efficiently interact with
D3/MAX2 and loses the ability to act as a receptor that triggers strigolactone
signalling in planta. These findings uncover a mechanism underlying the
allosteric activation of AtD14 by strigolactone hydrolysis into CLIM, and define
AtD14 as a non-canonical hormone receptor with dual functions to generate and
sense the active form of strigolactone.
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