PDBsum entry 5hpt

Ligase/transferase

PDB id: 5hpt

Contents

Protein chains

373 a.a.

76 a.a.

151 a.a.

Waters ×5

PDB id:

5hpt

Name:

Ligase/transferase

Title:

System-wide modulation of hect e3 ligases with selective ubiquitin variant probes: wwp1, ubv p2.3 and ubch7

Structure:

Nedd4-like e3 ubiquitin-protein ligase wwp1. Chain: a, d, g. Fragment: hect domain (unp residues 537-917). Synonym: atrophin-1-interacting protein 5,aip5,tgif-interacting ubiquitin ligase 1,tiul1,ww domain-containing protein 1. Engineered: yes. Ubiquitin variant p2.3. Chain: b, e, h. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: wwp1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: ube2l3, ubce7, ubch7.

Resolution:

2.84Å R-factor: 0.219 R-free: 0.237

Authors:

K.-P.Wu,B.A.Schulman

Key ref:

W.Zhang et al. (2016). System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes. Mol Cell, 62, 121-136. PubMed id: 26949039 DOI: 10.1016/j.molcel.2016.02.005

Date:

20-Jan-16 Release date: 16-Mar-16

Protein chains

Q9H0M0  (WWP1_HUMAN) -  NEDD4-like E3 ubiquitin-protein ligase WWP1 from Homo sapiens

Seq: 922 a.a.

Struc: 373 a.a.

Protein chains

P62987  (RL40_HUMAN) -  Ubiquitin-ribosomal protein eL40 fusion protein from Homo sapiens

Seq: 128 a.a.

Struc: 76 a.a.*

Protein chains

P68036  (UB2L3_HUMAN) -  Ubiquitin-conjugating enzyme E2 L3 from Homo sapiens

Seq: 154 a.a.

Struc: 151 a.a.

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, D, G: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: Chains C, F: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1016/j.molcel.2016.02.005

Mol Cell 62:121-136 (2016)

PubMed id: 26949039

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.

W.Zhang, K.P.Wu, M.A.Sartori, H.B.Kamadurai, A.Ordureau, C.Jiang, P.Y.Mercredi, R.Murchie, J.Hu, A.Persaud, M.Mukherjee, N.Li, A.Doye, J.R.Walker, Y.Sheng, Z.Hao, Y.Li, K.R.Brown, E.Lemichez, J.Chen, Y.Tong, J.W.Harper, J.Moffat, D.Rotin, B.A.Schulman, S.S.Sidhu.

ABSTRACT

No abstract given.