PDBsum entry 5hpk

Ligase

PDB id: 5hpk

Contents

Protein chains

372 a.a.

78 a.a.

Waters ×131

PDB id:

5hpk

Name:

Ligase

Title:

System-wide modulation of hect e3 ligases with selective ubiquitin variant probes: nedd4l and ubv nl.1

Structure:

E3 ubiquitin-protein ligase nedd4-like. Chain: a. Fragment: hect domain (unp residues 594-975). Synonym: nedd4.2,nedd4-2. Engineered: yes. Ubiquitin variant nl.1. Chain: b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: nedd4l, kiaa0439, nedl3. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.43Å R-factor: 0.212 R-free: 0.227

Authors:

K.-P.Wu,M.Mukherjee,P.Y.Mercredi,B.A.Schulman

Key ref:

W.Zhang et al. (2016). System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes. Mol Cell, 62, 121-136. PubMed id: 26949039 DOI: 10.1016/j.molcel.2016.02.005

Date:

20-Jan-16 Release date: 16-Mar-16

Protein chain

Q96PU5  (NED4L_HUMAN) -  E3 ubiquitin-protein ligase NEDD4-like from Homo sapiens

Seq: 975 a.a.

Struc: 372 a.a.*

Protein chain

P62987  (RL40_HUMAN) -  Ubiquitin-ribosomal protein eL40 fusion protein from Homo sapiens

Seq: 128 a.a.

Struc: 78 a.a.*

* PDB and UniProt seqs differ at 14 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

DOI no: 10.1016/j.molcel.2016.02.005

Mol Cell 62:121-136 (2016)

PubMed id: 26949039

System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes.

W.Zhang, K.P.Wu, M.A.Sartori, H.B.Kamadurai, A.Ordureau, C.Jiang, P.Y.Mercredi, R.Murchie, J.Hu, A.Persaud, M.Mukherjee, N.Li, A.Doye, J.R.Walker, Y.Sheng, Z.Hao, Y.Li, K.R.Brown, E.Lemichez, J.Chen, Y.Tong, J.W.Harper, J.Moffat, D.Rotin, B.A.Schulman, S.S.Sidhu.

ABSTRACT

No abstract given.