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UniProt functional annotation for Q9V2G1 | ||
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| UniProt code: Q9V2G1. |
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| Organism: | |
Pyrococcus abyssi (strain GE5 / Orsay). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
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| Function: | |
Catalyzes both the N1-methylation of guanosine and the C7- methylation of 4-demethylwyosine (imG-14) at position 37 in tRNA(Phe). {ECO:0000269|PubMed:20382657, ECO:0000269|PubMed:27852927}. |
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| Catalytic activity: | |
Reaction=guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)- methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:36899, Rhea:RHEA-COMP:10145, Rhea:RHEA-COMP:10147, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.228; Evidence={ECO:0000269|PubMed:20382657, ECO:0000269|PubMed:27852927}; |
| Catalytic activity: | |
Reaction=4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = H(+) + isowyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:53056, Rhea:RHEA-COMP:10164, Rhea:RHEA-COMP:13445, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315, ChEBI:CHEBI:136979; Evidence={ECO:0000269|PubMed:27852927, ECO:0000305|PubMed:20382657}; |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Miscellaneous: | |
Less efficient than Trm5b and appears to be selective for methylating tRNA(Phe). {ECO:0000305|PubMed:20382657}. |
| Similarity: | |
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family. {ECO:0000255|PROSITE-ProRule:PRU01021}. |
Annotations taken from UniProtKB at the EBI.