PDBsum entry 5g5l

RNA polymerase

PDB id: 5g5l

Contents

Protein chains

1480 a.a.

1174 a.a.

305 a.a.

58 a.a.

212 a.a.

100 a.a.

193 a.a.

131 a.a.

107 a.a.

69 a.a.

101 a.a.

43 a.a.

105 a.a.

145 a.a.

463 a.a.

Metals

_ZN ×7

PDB id:

5g5l

Name:

RNA polymerase

Title:

RNA polymerase i-rrn3 complex at 4.8 a resolution

Structure:

DNA-directed RNA polymerase i subunit rpa190. Chain: a. Synonym: DNA-directed RNA polymerase i 190 kda polypeptide, a190, DNA-directed RNA polymerase i largest subunit. Other_details: c-terminal flag-10xhis tag. DNA-directed RNA polymerase i subunit rpa135. Chain: b. Synonym: DNA-directed RNA polymerase i 135 kda polypeptide, a135, DNA-directed RNA polymerase i polypeptide 2, RNA polymerase i subunit

Source:

Saccharomyces cerevisiae. Bakers yeast. Organism_taxid: 4932. Strain: cb010. Atcc: 204508. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: ril.

Authors:

C.Engel,J.Plitzko,P.Cramer

Key ref:

C.Engel et al. (2016). RNA polymerase I-Rrn3 complex at 4.8 Å resolution. Nat Commun, 7, 12129. PubMed id: 27418309 DOI: 10.1038/ncomms12129

Date:

26-May-16 Release date: 27-Jul-16

Protein chain

P10964  (RPA1_YEAST) -  DNA-directed RNA polymerase I subunit RPA190 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1664 a.a.

Struc: 1480 a.a.

Protein chain

P22138  (RPA2_YEAST) -  DNA-directed RNA polymerase I subunit RPA135 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1203 a.a.

Struc: 1174 a.a.

Protein chain

P07703  (RPAC1_YEAST) -  DNA-directed RNA polymerases I and III subunit RPAC1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 335 a.a.

Struc: 305 a.a.

Protein chain

P50106  (RPA14_YEAST) -  DNA-directed RNA polymerase I subunit RPA14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 137 a.a.

Struc: 58 a.a.

Protein chain

P20434  (RPAB1_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 215 a.a.

Struc: 212 a.a.

Protein chain

P20435  (RPAB2_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 155 a.a.

Struc: 100 a.a.

Protein chain

P46669  (RPA43_YEAST) -  DNA-directed RNA polymerase I subunit RPA43 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 326 a.a.

Struc: 193 a.a.

Protein chain

P20436  (RPAB3_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 146 a.a.

Struc: 131 a.a.

Protein chain

P32529  (RPA12_YEAST) -  DNA-directed RNA polymerase I subunit RPA12 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 125 a.a.

Struc: 107 a.a.

Protein chain

P22139  (RPAB5_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 70 a.a.

Struc: 69 a.a.

Protein chain

P28000  (RPAC2_YEAST) -  DNA-directed RNA polymerases I and III subunit RPAC2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 142 a.a.

Struc: 101 a.a.

Protein chain

P40422  (RPAB4_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 70 a.a.

Struc: 43 a.a.

Protein chain

Q01080  (RPA49_YEAST) -  DNA-directed RNA polymerase I subunit RPA49 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 415 a.a.

Struc: 105 a.a.

Protein chain

P47006  (RPA34_YEAST) -  DNA-directed RNA polymerase I subunit RPA34 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 233 a.a.

Struc: 145 a.a.

Protein chain

P36070  (RRN3_YEAST) -  RNA polymerase I-specific transcription initiation factor RRN3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 627 a.a.

Struc: 463 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1038/ncomms12129

Nat Commun 7:12129 (2016)

PubMed id: 27418309

RNA polymerase I-Rrn3 complex at 4.8 Å resolution.

C.Engel, J.Plitzko, P.Cramer.

ABSTRACT

Transcription of ribosomal DNA by RNA polymerase I (Pol I) requires the initiation factor Rrn3. Here we report the cryo-EM structure of the Pol I-Rrn3 complex at 4.8 Å resolution. The structure reveals how Rrn3 binding converts an inactive Pol I dimer into an initiation-competent monomeric complex and provides insights into the mechanisms of Pol I-specific initiation and regulation.