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PDBsum entry 5fxy
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Transcription
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PDB id
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5fxy
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References listed in PDB file
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Key reference
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Title
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The structure of the core nurd repression complex provides insights into its interaction with chromatin.
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Authors
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C.J.Millard,
N.Varma,
A.Saleh,
K.Morris,
P.J.Watson,
A.R.Bottrill,
L.Fairall,
C.J.Smith,
J.W.Schwabe.
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Ref.
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Elife, 2016,
5,
e13941.
[DOI no: ]
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PubMed id
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Abstract
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The NuRD complex is a multi-protein transcriptional corepressor that couples
histone deacetylase and ATP-dependent chromatin remodelling activities. The
complex regulates the higher-order structure of chromatin, and has important
roles in the regulation of gene expression, DNA damage repair and cell
differentiation. HDACs 1 and 2 are recruited by the MTA1 corepressor to form the
catalytic core of the complex. The histone chaperone protein RBBP4, has
previously been shown to bind to the carboxy-terminal tail of MTA1. We show that
MTA1 recruits a second copy of RBBP4. The crystal structure reveals an extensive
interface between MTA1 and RBBP4. An EM structure, supported by SAXS and
crosslinking, reveals the architecture of the dimeric HDAC1:MTA1:RBBP4 assembly
which forms the core of the NuRD complex. We find evidence that in this complex
RBBP4 mediates interaction with histone H3 tails, but not histone H4, suggesting
a mechanism for recruitment of the NuRD complex to chromatin.
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