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PDBsum entry 5ftp
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References listed in PDB file
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Key reference
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Title
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Data collection with a tailored X-Ray beam size at 2.69 å wavelength (4.6 kev): sulfur sad phasing of cdc23(nterm).
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Authors
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M.Cianci,
M.R.Groves,
D.Barford,
T.R.Schneider.
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Ref.
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Acta Crystallogr D Struct Biol, 2016,
72,
403-412.
[DOI no: ]
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PubMed id
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Abstract
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The capability to reach wavelengths of up to 3.1 Å at the newly established
EMBL P13 beamline at PETRA III, the new third-generation synchrotron at DESY in
Hamburg, provides the opportunity to explore very long wavelengths to harness
the sulfur anomalous signal for phase determination. Data collection at λ =
2.69 Å (4.6 keV) allowed the crystal structure determination by sulfur SAD
phasing of Cdc23(Nterm), a subunit of the multimeric anaphase-promoting complex
(APC/C). At this energy, Cdc23(Nterm) has an expected Bijvoet ratio
〈|Fanom|〉/〈F〉 of 2.2%, with 282 residues, including six cysteines and
five methionine residues, and two molecules in the asymmetric unit (65.4 kDa;
12 Cys and ten Met residues). Selectively illuminating two separate portions of
the same crystal with an X-ray beam of 50 µm in diameter allowed crystal
twinning to be overcome. The crystals diffracted to 3.1 Å resolution, with
unit-cell parameters a = b = 61.2, c = 151.5 Å, and belonged to space group
P43. The refined structure to 3.1 Å resolution has an R factor of 18.7% and
an Rfree of 25.9%. This paper reports the structure solution, related methods
and a discussion of the instrumentation.
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