PDBsum entry 5fov

Hydrolase

PDB id: 5fov

Contents

Protein chains

227 a.a.

Ligands

ACE-PHE-HIS-THR-GLY-NH2 ×2

PO4 ×2

GOL

Waters ×634

References listed in PDB file

Key reference

• Title: Structure-Activity relationship of the peptide binding-Motif mediating the brca2:rad51 protein-Protein interaction.
• Authors: D.E.Scott, M.Marsh, T.L.Blundell, C.Abell, M.Hyvönen.
• Ref.: Febs Lett, 2016, 590, 1094-1102. [DOI no: 10.1002/1873-3468.12139]
• PubMed id: 26992456

Abstract

RAD51 is a recombinase involved in the homologous recombination of double-strand breaks in DNA. RAD51 forms oligomers by binding to another molecule of RAD51 via an 'FxxA' motif, and the same recognition sequence is similarly utilised to bind BRCA2. We have tabulated the effects of mutation of this sequence, across a variety of experimental methods and from relevant mutations observed in the clinic. We use mutants of a tetrapeptide sequence to probe the binding interaction, using both isothermal titration calorimetry and X-ray crystallography. Where possible, comparison between our tetrapeptide mutational study and the previously reported mutations is made, discrepancies are discussed and the importance of secondary structure in interpreting alanine scanning and mutational data of this nature is considered.