PDBsum entry 5fl7

Hydrolase

PDB id: 5fl7

Contents

Protein chains

485 a.a.

470 a.a.

267 a.a.

113 a.a.

16 a.a.

(+ 4 more) 73 a.a.

Ligands

ATP ×3

ADP ×2

Metals

_MG ×5

Waters ×17

PDB id:

5fl7

Name:

Hydrolase

Title:

Structure of the f1c10 complex from yarrowia lipolytica atp synthase

Structure:

Atp synthase subunit alpha. Chain: a, b, c. Synonym: atp synthase subunit alpha, mitochondrial. Atp synthase subunit beta. Chain: d, e, f. Synonym: atp synthase subunit beta, mitochondrial. Atp synthase subunit gamma chain, mitochondrial. Chain: g. Atp synthase delta chain, mitochondrial.

Source:

Yarrowia lipolytica. Organism_taxid: 4952. Organism_taxid: 4952

Resolution:

3.50Å R-factor: 0.275 R-free: 0.305

Authors:

K.Parey,M.Bublitz,T.Meier

Key ref:

A.Hahn et al. (2016). Structure of a Complete ATP Synthase Dimer Reveals the Molecular Basis of Inner Mitochondrial Membrane Morphology. Mol Cell, 63, 445-456. PubMed id: 27373333 DOI: 10.1016/j.molcel.2016.05.037

Date:

22-Oct-15 Release date: 29-Jun-16

Protein chains

Q6C326  (Q6C326_YARLI) -  ATP synthase subunit alpha, mitochondrial from Yarrowia lipolytica (strain CLIB 122 / E 150)

Seq: 536 a.a.

Struc: 485 a.a.

Protein chains

Q6CFT7  (Q6CFT7_YARLI) -  ATP synthase subunit beta, mitochondrial from Yarrowia lipolytica (strain CLIB 122 / E 150)

Seq: 509 a.a.

Struc: 470 a.a.

Protein chain

Q6C338  (Q6C338_YARLI) -  ATP synthase subunit gamma, mitochondrial from Yarrowia lipolytica (strain CLIB 122 / E 150)

Seq: 293 a.a.

Struc: 267 a.a.

Protein chain

Q6C877  (Q6C877_YARLI) -  ATP synthase subunit delta, mitochondrial from Yarrowia lipolytica (strain CLIB 122 / E 150)

Seq: 162 a.a.

Struc: 113 a.a.*

Protein chain

No UniProt id for this chain

Struc: 16 a.a.

Protein chains

Q37695  (ATP9_YARLI) -  ATP synthase subunit 9, mitochondrial from Yarrowia lipolytica (strain CLIB 122 / E 150)

Seq: 76 a.a.

Struc: 73 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains D, E, F: E.C.7.1.2.2 - H(+)-transporting two-sector ATPase.
• Reaction: ATP + H2O + 4 H⁺(in) = ADP + phosphate + 5 H⁺(out)

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + H2O + 4 × H(+)(in) = ADP + phosphate + 5 × H(+)(out)

• Enzyme class 3: Chains G, H: E.C.3.6.1.34 - Transferred entry: 7.1.2.2.
• Enzyme class 4: Chains K, L, M, N, O, P, Q, R, S, T: E.C.3.6.3.14 - Transferred entry: 7.1.2.2.
• Reaction: ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.molcel.2016.05.037

Mol Cell 63:445-456 (2016)

PubMed id: 27373333

Structure of a Complete ATP Synthase Dimer Reveals the Molecular Basis of Inner Mitochondrial Membrane Morphology.

A.Hahn, K.Parey, M.Bublitz, D.J.Mills, V.Zickermann, J.Vonck, W.Kühlbrandt, T.Meier.

ABSTRACT

No abstract given.