PDBsum entry 5f8v

Transferase

PDB id: 5f8v

Contents

Protein chains

(+ 1 more) 367 a.a.

343 a.a.

Waters ×749

PDB id:

5f8v

Name:

Transferase

Title:

Crystal structure of plp bound phosphoserine aminotransferase (psat) from trichomonas vaginalis

Structure:

Aminotransferase, class v family protein. Chain: a, e, b, c, d, f, g, h. Engineered: yes

Source:

Trichomonas vaginalis. Organism_taxid: 5722. Gene: tvag_224040. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.14Å R-factor: 0.210 R-free: 0.239

Authors:

R.K.Singh,S.Gourinath

Key ref:

R.K.Singh et al. (2016). Structural investigation and inhibitory response of halide on phosphoserine aminotransferase from Trichomonas vaginalis. Biochim Biophys Acta, 1860, 1508-1518. PubMed id: 27102280 DOI: 10.1016/j.bbagen.2016.04.013

Date:

09-Dec-15 Release date: 19-Oct-16

Protein chains

A2DW27  (A2DW27_TRIVA) -  phosphoserine transaminase from Trichomonas vaginalis (strain ATCC PRA-98 / G3)

Seq: 371 a.a.

Struc: 367 a.a.*

Protein chain

A2DW27  (A2DW27_TRIVA) -  phosphoserine transaminase from Trichomonas vaginalis (strain ATCC PRA-98 / G3)

Seq: 371 a.a.

Struc: 343 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, E, B, C, D, F, G, H: E.C.2.6.1.52 - phosphoserine transaminase.
• Reaction:
  1. O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate
  2. 4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4- phosphooxybutanoate + L-glutamate
• Cofactor: Pyridoxal 5'-phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.bbagen.2016.04.013

Biochim Biophys Acta 1860:1508-1518 (2016)

PubMed id: 27102280

Structural investigation and inhibitory response of halide on phosphoserine aminotransferase from Trichomonas vaginalis.

R.K.Singh, M.Mazumder, B.Sharma, S.Gourinath.

ABSTRACT

No abstract given.