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PDBsum entry 5f3x
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Structural protein/protein binding
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PDB id
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5f3x
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References listed in PDB file
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Key reference
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Title
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Mechanistic basis of organization of the harmonin/ush1c-Mediated brush border microvilli tip-Link complex.
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Authors
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J.Li,
Y.He,
Q.Lu,
M.Zhang.
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Ref.
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Dev Cell, 2016,
36,
179-189.
[DOI no: ]
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PubMed id
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Abstract
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Brush border microvilli are actin-based protrusions lining the apical surface of
epithelial cells in intestines and proximal tubules of kidneys. While brush
border microvilli resemble the relatively well-characterized stereocilia of hair
cells, the mechanistic basis of tip-link complex organization in microvilli is
poorly understood. Here, we have biochemically and structurally characterized
the following pairs of interactions: protocadherin 24 and Harmonin (also known
as USH1C or AIE-75), Harmonin and myosin VIIb (MYO7B), Harmonin and ANKS4B, and
ANKS4B and MYO7B. We show that Harmonin, ANKS4B, and MYO7B form a stable ternary
complex for anchoring microvilli tip-link cadherins. Despite having only
Harmonin in common, the microvilli and the stereocilia tip-link complexes are
formed via strikingly similar interaction modes. These results not only provide
insight into the mechanistic bases of brush border microvilli formation and
maintenance but may also be valuable for understanding some gut and/or kidney
diseases caused by perturbations of brush border microvilli structures.
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