PDBsum entry 5ewr

RNA binding protein

PDB id: 5ewr

Contents

Protein chain

122 a.a.

Waters ×42

PDB id:

5ewr

Name:

RNA binding protein

Title:

C merolae u4 snrnp protein snu13

Structure:

Box c/d snornp and u4 snrnp component snu13p. Chain: a. Engineered: yes

Source:

Cyanidioschyzon merolae. Red alga. Organism_taxid: 45157. Gene: cyme_cmp335c. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.35Å R-factor: 0.169 R-free: 0.220

Authors:

E.L.Garside,C.Black,S.Rader,A.M.Macmillan

Key ref:

C.S.Black et al. (2016). Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae. Protein Sci, 25, 911-916. PubMed id: 26833716

Date:

20-Nov-15 Release date: 20-Apr-16

Protein chain

M1VFX0  (M1VFX0_CYAM1) -  Ribonucloprotein from Cyanidioschyzon merolae (strain NIES-3377 / 10D)

Seq: 145 a.a.

Struc: 122 a.a.

Protein Sci 25:911-916 (2016)

PubMed id: 26833716

Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae.

C.S.Black, E.L.Garside, A.M.MacMillan, S.D.Rader.

ABSTRACT

Structural and functional analysis of proteins involved in pre-mRNA splicing is challenging because of the complexity of the splicing machinery, known as the spliceosome. Bioinformatic, proteomic, and biochemical analyses have identified a minimal spliceosome in the red alga Cyanidioschyzon merolae. This spliceosome consists of only 40 core proteins, compared to ∼ 70 in S. cerevisiae (yeast) and ∼ 150 in humans. We report the X-ray crystallographic analysis of C. merolae Snu13 (CmSnu13), a key component of the assembling spliceosome, and present evidence for conservation of Snu13 function in this algal splicing pathway. The near identity of CmSnu13's three-dimensional structure to yeast and human Snu13 suggests that C. merolae should be an excellent model system for investigating the structure and function of the conserved core of the spliceosome.