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UniProt functional annotation for P46959 | ||
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| UniProt code: P46959. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:10779558, PubMed:9851972). GCD14 is also required for repression of GCN4 mRNA translation by the upstream open reading frames (uORFs) under conditions of amino acid sufficiency (PubMed:9539420). {ECO:0000269|PubMed:10779558, ECO:0000269|PubMed:9539420, ECO:0000269|PubMed:9851972}. |
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| Catalytic activity: | |
Reaction=adenosine(58) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)- methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43152, Rhea:RHEA-COMP:10365, Rhea:RHEA-COMP:10366, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74491; EC=2.1.1.220; Evidence={ECO:0000255|PROSITE-ProRule:PRU00952}; |
| Subunit: | |
Heterotetramer; composed of two copies of TRM6/GCD10 and two copies of TRM61/GCD14. {ECO:0000269|PubMed:27582183, ECO:0000305|PubMed:9851972}. |
| Subcellular location: | |
Nucleus {ECO:0000269|PubMed:9851972}. |
| Miscellaneous: | |
Present with 7220 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family. {ECO:0000255|PROSITE-ProRule:PRU00952}. |
Annotations taken from UniProtKB at the EBI.