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PDBsum entry 5emb
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Protein transport
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PDB id
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5emb
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References listed in PDB file
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Key reference
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Title
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A molecular code for endosomal recycling of phosphorylated cargos by the snx27-Retromer complex.
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Authors
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T.Clairfeuille,
C.Mas,
A.S.Chan,
Z.Yang,
M.Tello-Lafoz,
M.Chandra,
J.Widagdo,
M.C.Kerr,
B.Paul,
I.Mérida,
R.D.Teasdale,
N.J.Pavlos,
V.Anggono,
B.M.Collins.
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Ref.
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Nat Struct Mol Biol, 2016,
23,
921-932.
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PubMed id
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Abstract
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Recycling of internalized receptors from endosomal compartments is essential for
the receptors' cell-surface homeostasis. Sorting nexin 27 (SNX27) cooperates
with the retromer complex in the recycling of proteins containing type I
PSD95-Dlg-ZO1 (PDZ)-binding motifs. Here we define specific acidic amino acid
sequences upstream of the PDZ-binding motif required for high-affinity
engagement of the human SNX27 PDZ domain. However, a subset of SNX27 ligands,
such as the β2adrenergic receptor and N-methyl-D-aspartate (NMDA)
receptor, lack these sequence determinants. Instead, we identified conserved
sites of phosphorylation that substitute for acidic residues and dramatically
enhance SNX27 interactions. This newly identified mechanism suggests a likely
regulatory switch for PDZ interaction and protein transport by the
SNX27-retromer complex. Defining this SNX27 binding code allowed us to classify
more than 400 potential SNX27 ligands with broad functional implications in
signal transduction, neuronal plasticity and metabolite transport.
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