PDBsum entry 5elk

RNA binding protein/RNA

PDB id: 5elk

Contents

Protein chain

121 a.a.

DNA/RNA

Metals

_ZN ×3

Waters ×19

PDB id:

5elk

Name:

RNA binding protein/RNA

Title:

Crystal structure of mouse unkempt zinc fingers 4-6 (znf4-6), bound to RNA

Structure:

Ring finger protein unkempt homolog. Chain: a. Fragment: unp residues 204-335. Synonym: zinc finger ccch domain-containing protein 5. Engineered: yes. RNA. Chain: r. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: unk, kiaa1753, zc3h5, zc3hdc5. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 10090

Resolution:

2.30Å R-factor: 0.182 R-free: 0.224

Authors:

M.Teplova,J.Murn,K.Zarnack,Y.Shi,D.J.Patel

Key ref:

J.Murn et al. (2016). Recognition of distinct RNA motifs by the clustered CCCH zinc fingers of neuronal protein Unkempt. Nat Struct Biol, 23, 16-23. PubMed id: 26641712 DOI: 10.1038/nsmb.3140

Date:

04-Nov-15 Release date: 09-Dec-15

Protein chain

Q8BL48  (UNK_MOUSE) -  RING finger protein unkempt homolog from Mus musculus

Seq: 810 a.a.

Struc: 121 a.a.

DNA/RNA chain

C-U-U-A-G-A 6 bases

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1038/nsmb.3140

Nat Struct Biol 23:16-23 (2016)

PubMed id: 26641712

Recognition of distinct RNA motifs by the clustered CCCH zinc fingers of neuronal protein Unkempt.

J.Murn, M.Teplova, K.Zarnack, Y.Shi, D.J.Patel.

ABSTRACT

Unkempt is an evolutionarily conserved RNA-binding protein that regulates translation of its target genes and is required for the establishment of the early bipolar neuronal morphology. Here we determined the X-ray crystal structure of mouse Unkempt and show that its six CCCH zinc fingers (ZnFs) form two compact clusters, ZnF1-3 and ZnF4-6, that recognize distinct trinucleotide RNA substrates. Both ZnF clusters adopt a similar overall topology and use distinct recognition principles to target specific RNA sequences. Structure-guided point mutations reduce the RNA binding affinity of Unkempt both in vitro and in vivo, ablate Unkempt's translational control and impair the ability of Unkempt to induce a bipolar cellular morphology. Our study unravels a new mode of RNA sequence recognition by clusters of CCCH ZnFs that is critical for post-transcriptional control of neuronal morphology.