PDBsum entry 5eg0

Transcription

PDB id: 5eg0

Contents

Protein chains

56 a.a.

61 a.a.

DNA/RNA

Waters ×29

PDB id:

5eg0

Name:

Transcription

Title:

Hoxb13-meis1 heterodimer bound to DNA

Structure:

Homeobox protein meis2. Chain: a. Fragment: unp residues 284-338. Synonym: meis1-related protein 1. Engineered: yes. DNA (5'- d(p Gp Tp Tp Gp Ap Cp Ap Gp Tp Tp Tp Tp Ap Cp Gp Ap Gp G)-3'). Chain: d. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: meis2, mrg1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta. Synthetic: yes. Synthetic construct.

Resolution:

3.10Å R-factor: 0.288 R-free: 0.322

Authors:

E.Morgunova,Y.Yin,A.Jolma,A.Popov,J.Taipale

Key ref:

E.Morgunova et al. Molecular basis of recognition of two distinct DNA se by a single transcription factor. To be published, . PubMed id: 40205063

Date:

26-Oct-15 Release date: 09-Nov-16

Protein chain

O14770  (MEIS2_HUMAN) -  Homeobox protein Meis2 from Homo sapiens

Seq: 477 a.a.

Struc: 56 a.a.*

Protein chain

Q92826  (HXB13_HUMAN) -  Homeobox protein Hox-B13 from Homo sapiens

Seq: 284 a.a.

Struc: 61 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-T-T-G-A-C-A-G-T-T-T-T-A-C-G-A-G-G 18 bases

C-C-T-C-G-T-A-A-A-A-C-T-G-T-C-A-A-C 18 bases