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UniProt functional annotation for V5LU01 | ||
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| UniProt code: V5LU01. |
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| Organism: | |
Ambrosia artemisiifolia (Short ragweed). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Asteroideae; Heliantheae alliance; Heliantheae; Ambrosia. |
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| Function: | |
Cysteine protease. Hydrolyzes casein and synthetic peptide Boc-Val-Leu-Lys-7-amino-4-methylcoumarin (Boc-VLK-AMC) in vitro. {ECO:0000269|PubMed:25865353, ECO:0000269|PubMed:27129273}. |
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| Activity regulation: | |
Activated by L-cysteine (PubMed:27129273). Inhibited by cysteine protease inhibitor E64 (PubMed:25865353, PubMed:27129273). Inhibited by cysteine/serine protease inhibitor leupeptin. Not inhibited by serine protease inhibitors 4-(2- aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) and phenylmethanesulfonyl fluoride (PMSF), metallo protease inhibitor bestatin or aspartic protease inhibitor pepstatin A (PubMed:27129273). {ECO:0000269|PubMed:25865353, ECO:0000269|PubMed:27129273}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is between 8 and 9. {ECO:0000269|PubMed:27129273}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:27129273}. |
| Tissue specificity: | |
Expressed in pollen (at protein and mRNA level). {ECO:0000269|PubMed:25865353}. |
| Ptm: | |
Autocatalytic proteolytic cleavage of N-terminal activation peptide. {ECO:0000269|PubMed:27129273}. |
| Ptm: | |
N-glycosylated. Glycosylation is not required for binding to IgE. {ECO:0000269|PubMed:25865353}. |
| Allergen: | |
Causes an allergic reaction in human. Binds to IgE in 54% of the 92 patients, including both Europeans and Americans, tested allergic to ragweed pollen. Purified native protein under non- denaturing conditions binds to IgE in 66% of the same 92 patients tested (PubMed:25865353). Binds to IgE of mice. In mice, the allergicity especially to the proteolytically active form of this protein leads to airway hyperresponsiveness, lung inflammation characterized by eosinophil and type 2 innate lymphoid cell (ILC2) infiltrates in bronchoalveolar lavages (BALs), and responses of the T- helper 2 (Th2) cells (PubMed:27129273). Causes activation of human basophils (PubMed:25865353). {ECO:0000269|PubMed:25865353, ECO:0000269|PubMed:27129273}. |
| Similarity: | |
Belongs to the peptidase C1 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.