PDBsum entry 5e95

Signaling protein/inhibitor

PDB id: 5e95

Contents

Protein chains

92 a.a.

168 a.a.

Ligands

GDP

Metals

_MG

Waters ×372

PDB id:

5e95

Name:

Signaling protein/inhibitor

Title:

Crystal structure of mb(ns1)/h-ras complex

Structure:

Mb(ns1). Chain: b. Engineered: yes. Gtpase hras. Chain: a. Fragment: unp residues 1-166. Synonym: h-ras-1,ha-ras,transforming protein p21,c-h-ras,p21ras. Engineered: yes

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Human. Gene: hras, hras1.

Resolution:

1.40Å R-factor: 0.164 R-free: 0.192

Authors:

R.R.Eguchi,F.Sha,A.Gupta,A.Koide,S.Koide

Key ref:

R.Spencer-Smith et al. (2017). Inhibition of RAS function through targeting an allosteric regulatory site. Nat Chem Biol, 13, 62-68. PubMed id: 27820802 DOI: 10.1038/nchembio.2231

Date:

14-Oct-15 Release date: 02-Nov-16

Protein chain

No UniProt id for this chain

Struc: 92 a.a.

Protein chain

P01112  (RASH_HUMAN) -  GTPase HRas from Homo sapiens

Seq: 189 a.a.

Struc: 168 a.a.

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GDP) corresponds exactly) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1038/nchembio.2231

Nat Chem Biol 13:62-68 (2017)

PubMed id: 27820802

Inhibition of RAS function through targeting an allosteric regulatory site.

R.Spencer-Smith, A.Koide, Y.Zhou, R.R.Eguchi, F.Sha, P.Gajwani, D.Santana, A.Gupta, M.Jacobs, E.Herrero-Garcia, J.Cobbert, H.Lavoie, M.Smith, T.Rajakulendran, E.Dowdell, M.N.Okur, I.Dementieva, F.Sicheri, M.Therrien, J.F.Hancock, M.Ikura, S.Koide, J.P.O'Bryan.

ABSTRACT

No abstract given.