PDBsum entry 5e5a

Protein binding/viral protein/DNA

PDB id: 5e5a

Contents

Protein chains

99 a.a.

79 a.a.

105 a.a.

98 a.a.

86 a.a.

14 a.a.

DNA/RNA

Metals

_MG ×4

Waters ×230

PDB id:

5e5a

Name:

Protein binding/viral protein/DNA

Title:

Crystal structure of the chromatin-tethering domain of human cytomegalovirus ie1 protein bound to the nucleosome core particle

Structure:

DNA (146-mer). Chain: i, j. Engineered: yes. Histone h3.2. Chain: a, e. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Humam. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Xenopus laevis. African clawed frog. Organism_taxid: 8355. Gene: hist1h2aj, loc494591.

Resolution:

2.81Å R-factor: 0.198 R-free: 0.243

Authors:

Q.Fang,P.Chen,M.Wang,J.Fang,N.Yang,G.Li,R.M.Xu

Key ref:

Q.Fang et al. (2016). Human cytomegalovirus ie1 protein alters the higher-O chromatin structure by targeting the acidic patch of nucleosome. Elife, 5, . PubMed id: 26812545

Date:

08-Oct-15 Release date: 03-Feb-16

Protein chains

P84233  (H32_XENLA) -  Histone H3.2 from Xenopus laevis

Seq: 136 a.a.

Struc: 99 a.a.*

Protein chain

P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis

Seq: 103 a.a.

Struc: 79 a.a.

Protein chains

P06897  (H2A1_XENLA) -  Histone H2A type 1 from Xenopus laevis

Seq: 130 a.a.

Struc: 105 a.a.*

Protein chains

P02281  (H2B11_XENLA) -  Histone H2B 1.1 from Xenopus laevis

Seq: 126 a.a.

Struc: 98 a.a.*

Protein chain

P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis

Seq: 103 a.a.

Struc: 86 a.a.

Protein chain

P03169  (VIE1_HCMVT) -  Immediate early protein IE1 from Human cytomegalovirus (strain Towne)

Seq: 491 a.a.

Struc: 14 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases