PDBsum entry 5dzd

Ligase/protein binding

PDB id: 5dzd

Contents

Protein chains

37 a.a.

39 a.a.

14 a.a.

11 a.a.

Ligands

UNX ×17

Waters ×60

PDB id:

5dzd

Name:

Ligase/protein binding

Title:

Crystal structure of ww4 domain of itch in complex with txnip peptide

Structure:

E3 ubiquitin-protein ligase itchy homolog. Chain: a, b. Fragment: unp residues 324-363. Synonym: itch, atrophin-1-interacting protein 4, aip4, nfe2- associated polypeptide 1, napp1. Engineered: yes. Thioredoxin-interacting protein. Chain: c, d. Fragment: unp residues 327-338.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: itch. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Other_details: synthetic

Resolution:

1.57Å R-factor: 0.176 R-free: 0.206

Authors:

Y.Liu,W.Tempel,A.Dong,C.Bountra,C.H.Arrowsmith,A.M.Edwards,J.Min, Structural Genomics Consortium (Sgc)

Key ref:

Y.Liu et al. Crystal structure of ww4 domain of itch in complex wi txnip peptide. To be published, .

Date:

25-Sep-15 Release date: 14-Oct-15

Supersedes:

4roi

Protein chain

Q96J02  (ITCH_HUMAN) -  E3 ubiquitin-protein ligase Itchy homolog from Homo sapiens

Seq: 903 a.a.

Struc: 37 a.a.

Protein chain

Q96J02  (ITCH_HUMAN) -  E3 ubiquitin-protein ligase Itchy homolog from Homo sapiens

Seq: 903 a.a.

Struc: 39 a.a.

Protein chain

Q9H3M7  (TXNIP_HUMAN) -  Thioredoxin-interacting protein from Homo sapiens

Seq: 391 a.a.

Struc: 13 a.a.*

Protein chain

Q9H3M7  (TXNIP_HUMAN) -  Thioredoxin-interacting protein from Homo sapiens

Seq: 391 a.a.

Struc: 11 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine