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PDBsum entry 5dup
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Immune system
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PDB id
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5dup
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Contents |
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195 a.a.
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208 a.a.
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209 a.a.
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PDB id:
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| Name: |
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Immune system
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Title:
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Influenza a virus h5 hemagglutinin globular head in complex with antibody avfluigg03
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Structure:
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Hemagglutinin. Chain: a. Fragment: globular head, unp residues 61-284. Engineered: yes. Avfluigg03 heavy chain. Chain: h. Engineered: yes. Avfluigg03 light chain. Chain: l.
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Source:
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Influenza a virus (a/anhui/1/2005(h5n1)). Organism_taxid: 370810. Strain: a/anhui/1/2005(h5n1). Gene: ha. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Homo sapiens. Organism_taxid: 9606.
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Resolution:
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3.05Å
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R-factor:
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0.207
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R-free:
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0.257
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Authors:
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T.Zuo,J.Sun,G.Wang,P.Zhou,X.Wang,L.Zhang
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Key ref:
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T.Zuo
et al.
(2015).
Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection.
Nat Commun,
6,
8855.
PubMed id:
DOI:
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Date:
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20-Sep-15
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Release date:
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16-Dec-15
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PROCHECK
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Headers
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References
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Q1WDM0
(Q1WDM0_9INFA) -
Hemagglutinin from Influenza A virus
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Seq:
Struc:
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567 a.a.
195 a.a.
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DOI no:
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Nat Commun
6:8855
(2015)
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PubMed id:
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Comprehensive analysis of antibody recognition in convalescent humans from highly pathogenic avian influenza H5N1 infection.
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T.Zuo,
J.Sun,
G.Wang,
L.Jiang,
Y.Zuo,
D.Li,
X.Shi,
X.Liu,
S.Fan,
H.Ren,
H.Hu,
L.Sun,
B.Zhou,
M.Liang,
P.Zhou,
X.Wang,
L.Zhang.
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ABSTRACT
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Understanding the mechanism of protective antibody recognition against highly
pathogenic avian influenza A virus H5N1 in humans is critical for the
development of effective therapies and vaccines. Here we report the crystal
structure of three H5-specific human monoclonal antibodies bound to the globular
head of hemagglutinin (HA) with distinct epitope specificities, neutralization
potencies and breadth. A structural and functional analysis of these epitopes
combined with those reported elsewhere identifies four major vulnerable sites on
the globular head of H5N1 HA. Chimeric and vulnerable site-specific mutant
pseudoviruses are generated to delineate broad neutralization specificities of
convalescent sera from two individuals who recovered from the infection with
H5N1 virus. Our results show that the four vulnerable sites on the globular head
rather than the stem region are the major neutralizing targets, suggesting that
during natural H5N1 infection neutralizing antibodies against the globular head
work in concert to provide protective antibody-mediated immunity.
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Links
