PDBsum entry 5dif

Transport protein

PDB id: 5dif

Contents

Protein chains

209 a.a.

130 a.a.

997 a.a.

15 a.a.

Ligands

GNP

GOL ×7

Metals

_MG

_CL

Waters ×660

PDB id:

5dif

Name:

Transport protein

Title:

Crystal structure of cpeb4 nes peptide in complex with crm1-ran-ranbp1

Structure:

Gtp-binding nuclear protein ran. Chain: a. Synonym: androgen receptor-associated protein 24,gtpase ran,ras-like protein tc4,ras-related nuclear protein. Engineered: yes. Ran-specific gtpase-activating protein 1. Chain: b. Fragment: randb1. Synonym: chromosome stability protein 20,perinuclear array-localized

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ran, ara24, ok/sw-cl.81. Expressed in: escherichia coli. Expression_system_taxid: 469008. Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292.

Resolution:

2.09Å R-factor: 0.172 R-free: 0.208

Authors:

H.Y.Fung,Y.M.Chook

Key ref:

H.Y.Fung et al. (2015). Structural determinants of nuclear export signal orie in binding crm1.. Elife, 4, . PubMed id: 26349033

Date:

31-Aug-15 Release date: 16-Sep-15

Protein chain

P62826  (RAN_HUMAN) -  GTP-binding nuclear protein Ran from Homo sapiens

Seq: 216 a.a.

Struc: 209 a.a.

Protein chain

P41920  (YRB1_YEAST) -  Ran-specific GTPase-activating protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 201 a.a.

Struc: 130 a.a.

Protein chain

P30822  (XPO1_YEAST) -  Exportin-1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1084 a.a.

Struc: 997 a.a.*

Protein chain

Q17RY0  (CPEB4_HUMAN) -  Cytoplasmic polyadenylation element-binding protein 4 from Homo sapiens

Seq: 729 a.a.

Struc: 15 a.a.

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chains B, C, D: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.