PDBsum entry 5d7l

Immune system

PDB id: 5d7l

Contents

Protein chains

262 a.a.

98 a.a.

242 a.a.

187 a.a.

241 a.a.

189 a.a.

Ligands

2LJ ×2

PDB id:

5d7l

Name:

Immune system

Title:

Structure of human mr1-5-op-ru in complex with human mav36 tcr

Structure:

Major histocompatibility complex class i-related gene protein. Chain: a, c. Fragment: extracellular domain residues 23-292. Synonym: mhc class i-related gene protein,class i histocompatibility antigen-like protein. Engineered: yes. Beta-2-microglobulin. Chain: b, f.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mr1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: b2m, cdabp0092, hdcma22p. Gene: trav/trav. Gene: trbv/trbc.

Resolution:

3.40Å R-factor: 0.283 R-free: 0.337

Authors:

A.N.Keller,J.Rossjohn

Key ref:

N.A.Gherardin et al. (2016). Diversity of T Cells Restricted by the MHC Class I-Related Molecule MR1 Facilitates Differential Antigen Recognition. Immunity, 44, 32-45. PubMed id: 26795251 DOI: 10.1016/j.immuni.2015.12.005

Date:

14-Aug-15 Release date: 27-Jan-16

Protein chain

Q95460  (HMR1_HUMAN) -  Major histocompatibility complex class I-related gene protein from Homo sapiens

Seq: 341 a.a.

Struc: 262 a.a.*

Protein chains

P61769  (B2MG_HUMAN) -  Beta-2-microglobulin from Homo sapiens

Seq: 119 a.a.

Struc: 98 a.a.*

Protein chain

Q95460  (HMR1_HUMAN) -  Major histocompatibility complex class I-related gene protein from Homo sapiens

Seq: 341 a.a.

Struc: 242 a.a.*

Protein chain

No UniProt id for this chain

Struc: 187 a.a.

Protein chains

No UniProt id for this chain

Struc: 241 a.a.

Protein chain

No UniProt id for this chain

Struc: 189 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DOI no: 10.1016/j.immuni.2015.12.005

Immunity 44:32-45 (2016)

PubMed id: 26795251

Diversity of T Cells Restricted by the MHC Class I-Related Molecule MR1 Facilitates Differential Antigen Recognition.

N.A.Gherardin, A.N.Keller, R.E.Woolley, J.Le Nours, D.S.Ritchie, P.J.Neeson, R.W.Birkinshaw, S.B.Eckle, J.N.Waddington, L.Liu, D.P.Fairlie, A.P.Uldrich, D.G.Pellicci, J.McCluskey, D.I.Godfrey, J.Rossjohn.

ABSTRACT

A characteristic of mucosal-associated invariant T (MAIT) cells is the expression of TRAV1-2(+) T cell receptors (TCRs) that are activated by riboflavin metabolite-based antigens (Ag) presented by the MHC-I related molecule, MR1. Whether the MR1-restricted T cell repertoire and associated Ag responsiveness extends beyond these cells remains unclear. Here, we describe MR1 autoreactivity and folate-derivative reactivity in a discrete subset of TRAV1-2(+) MAIT cells. This recognition was attributable to CDR3β loop-mediated effects within a consensus TRAV1-2(+) TCR-MR1-Ag footprint. Furthermore, we have demonstrated differential folate- and riboflavin-derivative reactivity by a diverse population of "atypical" TRAV1-2(-) MR1-restricted T cells. We have shown that TRAV1-2(-) T cells are phenotypically heterogeneous and largely distinct from TRAV1-2(+) MAIT cells. A TRAV1-2(-) TCR docks more centrally on MR1, thereby adopting a markedly different molecular footprint to the TRAV1-2(+) TCR. Accordingly, diversity within the MR1-restricted T cell repertoire leads to differing MR1-restricted Ag specificity.