PDBsum entry 5cyl

Cell adhesion

PDB id: 5cyl

Contents

Protein chains

(+ 1 more) 361 a.a.

342 a.a.

Waters ×298

PDB id:

5cyl

Name:

Cell adhesion

Title:

Crystal structure of the cupb6 tip adhesin from pseudomonas aeruginosa

Structure:

Fimbrial subunit cupb6. Chain: a, b, c, d, e, f, g, h. Fragment: unp residues 38-381. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 287. Gene: cupb6, pa4081. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.77Å R-factor: 0.240 R-free: 0.266

Authors:

M.Rasheed,J.A.Garnett,I.Perez-Dorado,S.J.Matthews

Key ref:

M.Rasheed et al. (2016). Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa. Biochim Biophys Acta, 1864, 1500-1505. PubMed id: 27481165 DOI: 10.1016/j.bbapap.2016.07.010

Date:

30-Jul-15 Release date: 05-Oct-16

Protein chains

Q9HWU7  (Q9HWU7_PSEAE) -  Fimbrial subunit CupB6 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 381 a.a.

Struc: 361 a.a.

Protein chain

Q9HWU7  (Q9HWU7_PSEAE) -  Fimbrial subunit CupB6 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 381 a.a.

Struc: 342 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/j.bbapap.2016.07.010

Biochim Biophys Acta 1864:1500-1505 (2016)

PubMed id: 27481165

Crystal structure of the CupB6 adhesive tip from the chaperone-usher family of pili from Pseudomonas aeruginosa.

M.Rasheed, J.Garnett, I.Pérez-Dorado, D.Muhl, A.Filloux, S.Matthews.

ABSTRACT

Pseudomonas aeruginosa is a Gram-negative opportunistic bacterial pathogen that can cause chronic infection of the lungs of cystic fibrosis patients. Chaperone-usher systems in P. aeruginosa are known to translocate and assemble adhesive pili on the bacterial surface and contribute to biofilm formation within the host. Here, we report the crystal structure of the tip adhesion subunit CupB6 from the cupB1-6 gene cluster. The tip domain is connected to the pilus via the N-terminal donor strand from the main pilus subunit CupB1. Although the CupB6 adhesion domain bears structural features similar to other CU adhesins it displays an unusual polyproline helix adjacent to a prominent surface pocket, which are likely the site for receptor recognition.