PDBsum entry 5ch2

Transferase

PDB id: 5ch2

Contents

Protein chains

479 a.a.

754 a.a.

Ligands

THR-LYS-ALA-ALA-ARG-MET-SER

SAH

Metals

_ZN ×8

Waters ×111

Obsolete entry

PDB id:

5ch2

Name:

Transferase

Title:

Crystal structure of an active polycomb repressive complex 2 in the basal state

Structure:

Putative polycomb protein eed. Chain: a. Engineered: yes. Putative polycomb protein ezh2,putative polycomb protein suz12. Chain: b. Engineered: yes. 20-mer peptide. Chain: e.

Source:

Chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719). Organism_taxid: 759272. Strain: dsm 1495 / cbs 144.50 / imi 039719. Gene: ctht_0029920. Expressed in: saccharomyces cerevisiae s288c. Expression_system_taxid: 559292. Chaetomium thermophilum. Gene: ctht_0053230, ctht_0006210.

Resolution:

2.71Å R-factor: 0.183 R-free: 0.249

Authors:

L.Jiao,X.Liu

Key ref:

L.Jiao and X.Liu (2015). Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2. Science, 350, aac4383. PubMed id: 26472914 DOI: 10.1126/science.aac4383

Date:

10-Jul-15 Release date: 28-Oct-15

Protein chain

G0S8H7  (G0S8H7_CHATD) -  Polycomb protein EED from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 565 a.a.

Struc: 479 a.a.

Protein chain

G0SDW4  (G0SDW4_CHATD) -  Histone-lysine N-methyltransferase EZH2 from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 1522 a.a.

Struc: 754 a.a.*

Protein chain

G0RYC6  (G0RYC6_CHATD) -  Polycomb protein SUZ12 from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 755 a.a.

Struc: 754 a.a.*

* PDB and UniProt seqs differ at 646 residue positions (black crosses)

DOI no: 10.1126/science.aac4383

Science 350:aac4383 (2015)

PubMed id: 26472914

Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2.

L.Jiao, X.Liu.

ABSTRACT

Polycomb repressive complex 2 (PRC2) catalyzes histone H3K27 trimethylation (H3K27me3), a hallmark of gene silencing. Here we report the crystal structures of an active PRC2 complex of 170 kilodaltons from the yeast Chaetomium thermophilum in both basal and stimulated states, which contain Ezh2, Eed, and the VEFS domain of Suz12 and are bound to a cancer-associated inhibiting H3K27M peptide and a S-adenosyl-l-homocysteine cofactor. The stimulated complex also contains an additional stimulating H3K27me3 peptide. Eed is engulfed by a belt-like structure of Ezh2, and Suz12(VEFS) contacts both of these two subunits to confer an unusual split active SET domain for catalysis. Comparison of PRC2 in the basal and stimulated states reveals a mobile Ezh2 motif that responds to stimulation to allosterically regulate the active site.