UniProt functional annotation for P63045



	UniProt functional annotation for P63045

UniProt code: P63045.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (Probable). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19690160). {ECO:0000250|UniProtKB:P63044, ECO:0000269|PubMed:19690160, ECO:0000305|PubMed:1331807}.

Subunit: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A (PubMed:19196426). This complex binds to CPLX1. Interacts with BVES and STX4. Interacts with VAPA and VAPB. Interacts with WDFY2, PRKCZ and PRKCI (By similarity). Interacts (via N-terminus) with KCNB1 (via N-terminus and C-terminus); stimulates the channel inactivation rate of KCNB1 (PubMed:19690160). Forms a complex with WDFY2 and PRKCZ (By similarity). Interacts with SEPT8; the interaction inhibits interaction of VAMP2 with SYP (PubMed:19196426). Interacts with SYP; the interaction is inhibited by interaction with SEPT8 (PubMed:19196426). Interacts with PICALM (By similarity). Interacts with alpha-synuclein/SNCA (By similarity). Interacts with STX3 (By similarity). {ECO:0000250|UniProtKB:P63027, ECO:0000250|UniProtKB:P63044, ECO:0000269|PubMed:19196426, ECO:0000269|PubMed:19690160}.

Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P63027}; Single-pass type IV membrane protein {ECO:0000255}. Cell membrane {ECO:0000269|PubMed:19690160}. Note=Colocalizes with PRKCZ and WDFY2 in intracellular vesicles. {ECO:0000250|UniProtKB:P63027}.

Tissue specificity: Nervous system specific. A higher level expression is seen in the brain as compared to the spinal cord (PubMed:2472388). Expressed in hippocampal neurons (PubMed:19196426). {ECO:0000269|PubMed:19196426, ECO:0000269|PubMed:2472388}.

Ptm: Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2. {ECO:0000250|UniProtKB:P63027}.

Ptm: (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which hydrolyzes the 76-Gln-|-Phe-77 bond and inhibits neurotransmitter release (PubMed:1331807). {ECO:0000269|PubMed:1331807}.

Ptm: (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 59-Lys-|-Leu-60 bond and inhibits neurotransmitter release (PubMed:8175689). {ECO:0000269|PubMed:8175689}.

Ptm: (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 58-Gln-|-Lys-59 bond and probably inhibits neurotransmitter release (PubMed:8505288). {ECO:0000269|PubMed:8505288, ECO:0000305|PubMed:8175689}.

Ptm: (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type G (BoNT/G, botG) which hydrolyzes the 81-Ala-|-Ala-82 bond and probably inhibits neurotransmitter release (PubMed:8505288). {ECO:0000269|PubMed:7910017}.

Ptm: (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which hydrolyzes the 66-Arg-|-Ala-67 bond and probably inhibits neurotransmitter release (PubMed:28770820). It remains unknown whether BoNT/X is ever produced, or what organisms it targets. {ECO:0000269|PubMed:28770820}.

Ptm: (Microbial infection) Targeted and hydrolyzed by C.tetani toxin (tetX) which hydrolyzes the 76-Gln-|-Phe-77 bond and inhibits neurotransmitter release (PubMed:1331807). {ECO:0000269|PubMed:1331807, ECO:0000305|PubMed:8175689}.

Similarity: Belongs to the synaptobrevin family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (Probable). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:19690160). {ECO:0000250\|UniProtKB:P63044, ECO:0000269\|PubMed:19690160, ECO:0000305\|PubMed:1331807}.


Subunit:		Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A (PubMed:19196426). This complex binds to CPLX1. Interacts with BVES and STX4. Interacts with VAPA and VAPB. Interacts with WDFY2, PRKCZ and PRKCI (By similarity). Interacts (via N-terminus) with KCNB1 (via N-terminus and C-terminus); stimulates the channel inactivation rate of KCNB1 (PubMed:19690160). Forms a complex with WDFY2 and PRKCZ (By similarity). Interacts with SEPT8; the interaction inhibits interaction of VAMP2 with SYP (PubMed:19196426). Interacts with SYP; the interaction is inhibited by interaction with SEPT8 (PubMed:19196426). Interacts with PICALM (By similarity). Interacts with alpha-synuclein/SNCA (By similarity). Interacts with STX3 (By similarity). {ECO:0000250\|UniProtKB:P63027, ECO:0000250\|UniProtKB:P63044, ECO:0000269\|PubMed:19196426, ECO:0000269\|PubMed:19690160}.
Subcellular location:		Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:P63027}; Single-pass type IV membrane protein {ECO:0000255}. Cell membrane {ECO:0000269\|PubMed:19690160}. Note=Colocalizes with PRKCZ and WDFY2 in intracellular vesicles. {ECO:0000250\|UniProtKB:P63027}.
Tissue specificity:		Nervous system specific. A higher level expression is seen in the brain as compared to the spinal cord (PubMed:2472388). Expressed in hippocampal neurons (PubMed:19196426). {ECO:0000269\|PubMed:19196426, ECO:0000269\|PubMed:2472388}.
Ptm:		Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2. {ECO:0000250\|UniProtKB:P63027}.
Ptm:		(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which hydrolyzes the 76-Gln-\|-Phe-77 bond and inhibits neurotransmitter release (PubMed:1331807). {ECO:0000269\|PubMed:1331807}.
Ptm:		(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which hydrolyzes the 59-Lys-\|-Leu-60 bond and inhibits neurotransmitter release (PubMed:8175689). {ECO:0000269\|PubMed:8175689}.
Ptm:		(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 58-Gln-\|-Lys-59 bond and probably inhibits neurotransmitter release (PubMed:8505288). {ECO:0000269\|PubMed:8505288, ECO:0000305\|PubMed:8175689}.
Ptm:		(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type G (BoNT/G, botG) which hydrolyzes the 81-Ala-\|-Ala-82 bond and probably inhibits neurotransmitter release (PubMed:8505288). {ECO:0000269\|PubMed:7910017}.
Ptm:		(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which hydrolyzes the 66-Arg-\|-Ala-67 bond and probably inhibits neurotransmitter release (PubMed:28770820). It remains unknown whether BoNT/X is ever produced, or what organisms it targets. {ECO:0000269\|PubMed:28770820}.
Ptm:		(Microbial infection) Targeted and hydrolyzed by C.tetani toxin (tetX) which hydrolyzes the 76-Gln-\|-Phe-77 bond and inhibits neurotransmitter release (PubMed:1331807). {ECO:0000269\|PubMed:1331807, ECO:0000305\|PubMed:8175689}.
Similarity:		Belongs to the synaptobrevin family. {ECO:0000305}.