PDBsum entry 5c9z

Transferase/transferase inhibitor

PDB id: 5c9z

Contents

Protein chains

625 a.a.

313 a.a.

Ligands

SFG

4Z2

GOL

Waters ×123

PDB id:

5c9z

Name:

Transferase/transferase inhibitor

Title:

Crystal structure of prmt5:mep50 with epz015866 and sinefungin

Structure:

Protein arginine n-methyltransferase 5. Chain: a. Synonym: 72 kda icln-binding protein,histone-arginine n- methyltransferase prmt5,jak-binding protein 1,shk1 kinase-binding protein 1 homolog,skb1hs. Engineered: yes. Mutation: yes. Methylosome protein 50. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: prmt5, hrmt1l5, ibp72, jbp1, skb1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: wdr77, mep50, wd45, hkmt1069, nbla10071.

Resolution:

2.36Å R-factor: 0.239 R-free: 0.288

Authors:

P.A.Boriack-Sjodin

Key ref:

P.A.Boriack-Sjodin Crystal structure of prmt5/mep50 complex with sinefun 15866. To be published, .

Date:

29-Jun-15 Release date: 29-Jun-16

Protein chain

O14744  (ANM5_HUMAN) -  Protein arginine N-methyltransferase 5 from Homo sapiens

Seq: 637 a.a.

Struc: 625 a.a.

Protein chain

Q9BQA1  (MEP50_HUMAN) -  Methylosome protein WDR77 from Homo sapiens

Seq: 342 a.a.

Struc: 313 a.a.

Enzyme reactions

• Enzyme class: Chain A: E.C.2.1.1.320 - type Ii protein arginine methyltransferase.
• Reaction: L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L- homocysteine + 2 H⁺

L-arginyl-[protein] + 2 × S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 × S-adenosyl-L- homocysteine + 2 × H(+) (Bound ligand (Het Group name = SFG) matches with 51.43% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site