UniProt functional annotation for Q9UBF8



	UniProt functional annotation for Q9UBF8

UniProt code: Q9UBF8.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity). {ECO:0000250|UniProtKB:O08561, ECO:0000269|PubMed:10559940, ECO:0000269|PubMed:11277933, ECO:0000269|PubMed:12749687, ECO:0000269|PubMed:9405935}.

Function: (Microbial infection) Plays an essential role in Aichi virus RNA replication (PubMed:22124328, PubMed:27989622, PubMed:22258260). Recruited by ACBD3 at the viral replication sites (PubMed:22124328, PubMed:27989622). {ECO:0000269|PubMed:22124328, ECO:0000269|PubMed:22258260, ECO:0000269|PubMed:27989622}.

Function: (Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV. {ECO:0000269|PubMed:22253445}.

Catalytic activity: Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+); Xref=Rhea:RHEA:19877, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57880, ChEBI:CHEBI:58178, ChEBI:CHEBI:456216; EC=2.7.1.67; Evidence={ECO:0000269|PubMed:11277933, ECO:0000269|PubMed:9405935}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19878; Evidence={ECO:0000305|PubMed:11277933};

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:11277933}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:11277933};

Activity regulation: Inhibited by wortmannin and adenosine (PubMed:11277933). Increased kinase activity upon interaction with NCS1/FREQ (By similarity). {ECO:0000250|UniProtKB:O02810, ECO:0000269|PubMed:11277933}.

Activity regulation: (Microbial infection) Activated by Aichi virus protein 3A, this activation is sensitized by ACBD3. {ECO:0000269|PubMed:24672044, ECO:0000269|PubMed:27989622}.

Biophysicochemical properties: Kinetic parameters: KM=1.0 mM for PtdIns {ECO:0000269|PubMed:11277933}; KM=1.0 mM for ATP {ECO:0000269|PubMed:11277933};

Subunit: Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (PubMed:17555535). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3 (PubMed:23572552, PubMed:27009356, PubMed:27989622, PubMed:22124328, PubMed:22258260). Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (PubMed:23572552). Interacts with GGA2 (via VHS domain); the interaction is important for PI4KB location at the Golgi apparatus membrane (PubMed:28289207). {ECO:0000250|UniProtKB:O08561, ECO:0000269|PubMed:17555535, ECO:0000269|PubMed:22124328, ECO:0000269|PubMed:22258260, ECO:0000269|PubMed:23572552, ECO:0000269|PubMed:27009356, ECO:0000269|PubMed:27989622, ECO:0000269|PubMed:28289207}.

Subunit: (Microbial infection) Interacts with Aichi virus protein 3A. Part of a complex Aichi virus protein 3A/ACBD3/PI4KB that allows the synthesis of PI4P at the viral RNA replication sites. {ECO:0000269|PubMed:22124328, ECO:0000269|PubMed:22258260, ECO:0000269|PubMed:27989622}.

Subcellular location: Endomembrane system. Mitochondrion outer membrane; Peripheral membrane protein. Rough endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus {ECO:0000269|PubMed:22124328}. Golgi apparatus membrane {ECO:0000269|PubMed:24672044, ECO:0000269|PubMed:27009356, ECO:0000269|PubMed:28289207}. Cytoplasm, perinuclear region. Note=Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5- bisphosphate (PIP2) on the Golgi complex. Recruited to the Golgi apparatus membrane by ACBD3 (PubMed:24672044, PubMed:27009356, PubMed:28289207). GGA2 is also involved in the recruitment (PubMed:28289207). {ECO:0000269|PubMed:24672044, ECO:0000269|PubMed:27009356, ECO:0000269|PubMed:28289207}.

Tissue specificity: Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver. {ECO:0000269|PubMed:9020160, ECO:0000269|PubMed:9405935, ECO:0000269|PubMed:9405938}.

Similarity: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. {ECO:0000305}.

Sequence caution: Sequence=BAA21661.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=EAW53450.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity). {ECO:0000250\|UniProtKB:O08561, ECO:0000269\|PubMed:10559940, ECO:0000269\|PubMed:11277933, ECO:0000269\|PubMed:12749687, ECO:0000269\|PubMed:9405935}.



Function:		(Microbial infection) Plays an essential role in Aichi virus RNA replication (PubMed:22124328, PubMed:27989622, PubMed:22258260). Recruited by ACBD3 at the viral replication sites (PubMed:22124328, PubMed:27989622). {ECO:0000269\|PubMed:22124328, ECO:0000269\|PubMed:22258260, ECO:0000269\|PubMed:27989622}.



Function:		(Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV. {ECO:0000269\|PubMed:22253445}.


Catalytic activity:		Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+); Xref=Rhea:RHEA:19877, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57880, ChEBI:CHEBI:58178, ChEBI:CHEBI:456216; EC=2.7.1.67; Evidence={ECO:0000269\|PubMed:11277933, ECO:0000269\|PubMed:9405935}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19878; Evidence={ECO:0000305\|PubMed:11277933};
Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269\|PubMed:11277933}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269\|PubMed:11277933};
Activity regulation:		Inhibited by wortmannin and adenosine (PubMed:11277933). Increased kinase activity upon interaction with NCS1/FREQ (By similarity). {ECO:0000250\|UniProtKB:O02810, ECO:0000269\|PubMed:11277933}.
Activity regulation:		(Microbial infection) Activated by Aichi virus protein 3A, this activation is sensitized by ACBD3. {ECO:0000269\|PubMed:24672044, ECO:0000269\|PubMed:27989622}.
Biophysicochemical properties:		Kinetic parameters: KM=1.0 mM for PtdIns {ECO:0000269\|PubMed:11277933}; KM=1.0 mM for ATP {ECO:0000269\|PubMed:11277933};
Subunit:		Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (PubMed:17555535). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3 (PubMed:23572552, PubMed:27009356, PubMed:27989622, PubMed:22124328, PubMed:22258260). Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (PubMed:23572552). Interacts with GGA2 (via VHS domain); the interaction is important for PI4KB location at the Golgi apparatus membrane (PubMed:28289207). {ECO:0000250\|UniProtKB:O08561, ECO:0000269\|PubMed:17555535, ECO:0000269\|PubMed:22124328, ECO:0000269\|PubMed:22258260, ECO:0000269\|PubMed:23572552, ECO:0000269\|PubMed:27009356, ECO:0000269\|PubMed:27989622, ECO:0000269\|PubMed:28289207}.
Subunit:		(Microbial infection) Interacts with Aichi virus protein 3A. Part of a complex Aichi virus protein 3A/ACBD3/PI4KB that allows the synthesis of PI4P at the viral RNA replication sites. {ECO:0000269\|PubMed:22124328, ECO:0000269\|PubMed:22258260, ECO:0000269\|PubMed:27989622}.
Subcellular location:		Endomembrane system. Mitochondrion outer membrane; Peripheral membrane protein. Rough endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus {ECO:0000269\|PubMed:22124328}. Golgi apparatus membrane {ECO:0000269\|PubMed:24672044, ECO:0000269\|PubMed:27009356, ECO:0000269\|PubMed:28289207}. Cytoplasm, perinuclear region. Note=Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5- bisphosphate (PIP2) on the Golgi complex. Recruited to the Golgi apparatus membrane by ACBD3 (PubMed:24672044, PubMed:27009356, PubMed:28289207). GGA2 is also involved in the recruitment (PubMed:28289207). {ECO:0000269\|PubMed:24672044, ECO:0000269\|PubMed:27009356, ECO:0000269\|PubMed:28289207}.
Tissue specificity:		Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver. {ECO:0000269\|PubMed:9020160, ECO:0000269\|PubMed:9405935, ECO:0000269\|PubMed:9405938}.
Similarity:		Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. {ECO:0000305}.
Sequence caution:		Sequence=BAA21661.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=EAW53450.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};