PDBsum entry 5c3n

Hydrolase

PDB id: 5c3n

Contents

Protein chains

301 a.a.

PDB id:

5c3n

Name:

Hydrolase

Title:

Crystal structure of mers coronavirus main protease in spacegroup c2221

Structure:

Orf1a protein. Chain: a, b. Fragment: unp residues 3248-3553. Engineered: yes

Source:

Middle east respiratory syndrome coronavirus. Organism_taxid: 1335626. Gene: orf1ab. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

3.00Å R-factor: 0.221 R-free: 0.282

Authors:

C.Y.Chou,S.C.Cheng

Key ref:

B.L.Ho et al. (2015). Critical Assessment of the Important Residues Involved in the Dimerization and Catalysis of MERS Coronavirus Main Protease. Plos One, 10, e0144865. PubMed id: 26658006 DOI: 10.1371/journal.pone.0144865

Date:

17-Jun-15 Release date: 30-Dec-15

Protein chains

V9TU05  (V9TU05_MERS) -  Orf1a from Middle East respiratory syndrome-related coronavirus

Seq: 4391 a.a.

Struc: 301 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

DOI no: 10.1371/journal.pone.0144865

Plos One 10:e0144865 (2015)

PubMed id: 26658006

Critical Assessment of the Important Residues Involved in the Dimerization and Catalysis of MERS Coronavirus Main Protease.

B.L.Ho, S.C.Cheng, L.Shi, T.Y.Wang, K.I.Ho, C.Y.Chou.

ABSTRACT

No abstract given.